Besides the more active habits of the males, their earlier emergence from the cocoon, and in some cases their frequenting more open stations, other causes may be assigned for an apparent or real difference in the proportional numbers of the sexes of Lepidoptera, when captured in the imago state, and when reared from the egg or caterpillar state. I hear from Professor Canestrini, that it is believed by many breeders in Italy, that the female caterpillar of the silk-moth suffers more from the recent disease than the male; and Dr. Staudinger informs me that in rearing Lepidoptera more females die in the cocoon than males. With many species the female caterpillar is larger than the male, and a collector would naturally choose the finest specimens, and thus unintentionally collect a larger number of females. Three collectors have told me that this was their practice; but Dr. Wallace is sure that most collectors take all the specimens which they can find of the rarer kinds, which alone are worth the trouble of rearing. Birds when surrounded by caterpillars would probably devour the largest; and Professor Canestrini informs me that in Italy some breeders believe, though on insufficient evidence, that in the first broods of the Ailanthus silk-moth, the wasps destroy a larger number of the female than of the male caterpillars. Dr. Wallace further remarks that female caterpillars, from being larger than the males, require more time for their development, and consume more food and moisture: and thus they would be exposed during a longer time to danger from ichneumons, birds, etc., and in times of scarcity would perish in greater numbers. Hence it appears quite possible that in a state of nature, fewer female Lepidoptera may reach maturity than males; and for our special object we are concerned with their relative numbers at maturity, when the sexes are ready to propagate their kind.