{10}OIL GLOBULES.--It has been established by Pfitzer that starch and sugar, as assimilation products, are replaced by oil in the cells of diatoms ("da bekannlich Staerke und Zucker bei den Bacillariaceen nicht nachzuweisen sind"). The oil drops are more or less numerous, of various sizes, and are found in the cytoplasma, the cell-sap, and sometimes the chromatophores. Mereschkowsky describes certain globules as elæoplasts, which he divides into four kinds according to their number and position. Whether all of these are oil globules is a question not yet determined. Other bodies, known as "Buetschli granules," or volutin, and described as "little blisters filled with a tolerably robust refractive substance," are considered by Lauterborn to be a nitrogen reserve store. They are found in the cytoplasma, or in the cell-sap, and can be fixed in picric acid and stained in methylene blue. NOTE.--For a discussion of the morphology of diatoms and a valuable résumé of the investigations of Buetschli, Karsten, Lauterborn, Mereschkowsky, Mueller, Pfitzer, Schuett, and others, the student is referred to "Der Bau der Diatomzelle," by Dr. Otto Heinzerling, in "Bibliotheca Botanica," 1908. CELL DIVISION The growth of diatoms follows the usual method of cell division as described by Sachs (Text Book of Botany, 2nd ed., p. 16): "The nucleus of a cell which is about to divide becomes broader, assuming the form of a biconcave lens, and its nucleolus breaks up into irregular granules which together with its other granular contents begin to form a nuclear disc in the equatorial plane. A delicate striation is now apparent in what is becoming the long axis of the nucleus, at right angles to the nuclear disc, and the characteristic nuclear spindle is gradually produced. The nuclear disc splits into two halves lying side by side, each of which travels to the corresponding pole of the nucleus; thus two nuclei are constituted which are connected by fibrillæ." The cell-wall and the chromatophore bands divide, each nucleus passes to the centre, and two new cells are formed. In the meantime, to permit of this division, the two siliceous valves separate, the girdle bands slipping over each other, and opposite the larger or enclosing valve a new valve is formed, the girdle band of which is seen later within the girdle of the mother valve. Opposite the smaller valve of the original cell and adjoining the new valve, another valve is formed which also produces a girdle within the girdle of the smaller valve. As a result of division we have, therefore, the valves of the original, or mother cell, the two new valves and four girdle bands. (Pl. 40, Figs. 18 and 19.)