As for the species of sturgeons which occur in the Canadian lakes, I know only three from personal examination, one of which was obtained in Lake Superior, at Michipicotin, another at the Pic, and the third at the Sault; though I know that they occur in all other Canadian lakes, yet it remains to be ascertained how the species said to be so common in Lake Huron, compared with those of Lake Superior, and with those in the other great lakes and the St Lawrence itself. As for the Atlantic species, ascending the rivers of the United States west and south of Cape Cod, I know them to differ from those of the lakes, at least from those which I possess from Lake Superior. The number of species of this interesting family which occur in the United States is, at all events, far greater than would be supposed from an examination of the published records. Upon close comparison of the specimens in my collection from different parts of the country, and in different museums, as those of the Natural History Society of Boston, of Salem, of the Lyceum of New York, my assistant, Mr Charles Giran, and myself, have discovered several species not described. For this comparison I was the better prepared, as I had an opportunity in former years of studying almost all the European species in a fresh condition, during a prolonged visit in Vienna. II.--_Fishes of Lake Superior compared with those of the other great Canadian Lakes._ Besides the interest there is everywhere in studying the living animals of a new country, there is a particular interest to a naturalist in ascertaining their peculiar geographical distribution, and their true affinities with those of other countries. It is only by following such a course, that we can hope to arrive at any exact results as to their origin. In this respect the fresh-water animals have a peculiar interest, as from the element they inhabit, they are placed under exceptional circumstances. Marine animals, as well as those inhabiting dry land, seem to have a boundless opportunity before them to spread over large parts of the earth's surface, and their locomotive powers would generally be sufficient to carry them almost anywhere; but they do not avail themselves of the possibility; notwithstanding their facilities for locomotion, they for the most part remain within very narrow limits, using their liberty rather to keep within certain definite bounds. This tendency of the higher animals especially, to keep within well-ascertained limits, is perhaps the strongest evidence that there is a natural connection between the external world and the organised beings living upon the present surface of our globe. The laws which regulate these relations, and those of geographical distribution in particular, have already been ascertained to a certain extent, and will receive additional evidence from the facts recorded during our journey.