E-text prepared by Suzanne Lybarger, Brian Janes, Josephine Paolucci, and the Project Gutenberg Online Distributed Proofreading Team (https://www.pgdp.net) Note: Project Gutenberg also has an HTML version of this file which includes the original illustrations. See 27713-h.htm or 27713-h.zip: (https://www.gutenberg.org/dirs/2/7/7/1/27713/27713-h/27713-h.htm) or (https://www.gutenberg.org/dirs/2/7/7/1/27713/27713-h.zip) Transcriber's note: Text enclosed by tilde marks was in bold face in the original (~bold~). Text enclosed by underscore marks is in italics (_italics_). The italic designation for single italized letters (such as variables in equations) and "foreign" abbreviations has been omitted for ease of reading. In numbers, equations, and chemical formulas, an underscore indicates that the following term enclosed within curly brackets is a subscript. Examples: CO_{2}, H_{2}SO_{4}. A carat character indicates that the following term enclosed within curly brackets is a superscript. For example, 11.1^{3} is 11.1 to the third power. Minor typographical errors have been corrected. THE ELEMENTS OF BACTERIOLOGICAL TECHNIQUE A Laboratory Guide for Medical, Dental, and Technical Students by J. W. H. EYRE, M.D., M.S., F.R.S. (EDIN.) Director of the Bacteriological Department of Guy's Hospital, London, and Lecturer on Bacteriology in the Medical and Dental Schools; formerly Lecturer on Bacteriology at Charing Cross Hospital Medical School, and Bacteriologist to Charing Cross Hospital; sometime Hunterian Professor, Royal College of Surgeons, England Second Edition Rewritten and Enlarged Philadelphia and London W. B. Saunders Company 1913 Copyright, 1902, by W. B. Saunders and Company Revised, entirely reset, reprinted, and recopyrighted July, 1913 Copyright, 1913, by W. B. Saunders Company Registered at Stationers' Hall, London, England Printed in America Press of W. B. Saunders Company Philadelphia TO THE MEMORY OF JOHN WICHENFORD WASHBOURN, C.M.G., M.D., F.R.C.P. Physician to Guy's Hospital and Lecturer on Bacteriology in the Medical School, and Physician to the London Fever Hospital MY TEACHER, FRIEND, AND CO-WORKER PREFACE TO THE SECOND EDITION