Arborescent: Branched or tree-like, beset with branched hair-like extensions (Fig. 150, e). (C) _Area of Liquefaction_ (if present).-- Crateriform: A saucer-shaped liquefaction of the gelatine (Fig. 151, f). Saccate: Shape of an elongated sack, tubular cylindrical (Fig. 151, g). Infundibuliform: Shape of a funnel, conical (Fig. 151, h). Napiform: Shape of a turnip (Fig. 151, j). Fusiform: Outline of a parsnip, narrow at either end, broadest below the surface (Fig. 151, k). Stratiform: Liquefaction extending to the walls of the tube and downward horizontally (Fig. 151, l). (D) _Character of the Liquefied Gelatine._-- 1. Pellicle on surface. 2. Uniformly turbid. 3. Granular. 4. Mainly clear, but containing flocculi. 5. Deposit at apex of liquefied portion. (E) _Production of Gas Bubbles._ ~Shake Cultures.~-- 1. Presence or absence of liquefaction. 2. Production of gas bubbles. 3. Bulk of growth at the surface--aerobic. 4. Bulk of growth in depths--anaerobic. ~Fluid Media.~ ~1. Surface of the Liquid.~-- Presence or absence of froth due to gas bubbles. Presence or absence of pellicle formation. Character of pellicle. ~2. Body of the Liquid.~-- Uniformly turbid. Flocculi in suspension. Granules in suspension. Clear, with precipitate at bottom of tube. Colouration of fluid, presence or absence of. ~3. Precipitate.~-- Character. Amount. Colour. ~Carbohydrate Media.~-- Growth. Reaction. Gas formation. Coagulation or not of serum albumen (when serum water media are employed). ~Litmus Milk Cultivations.~-- {Unaltered. 1. Reaction: {Acid. {Alkaline. 2. Odour. 3. Formation of gas. {Unaltered. 4. Consistency: {Peptonised (character of solution). {Coagulated. {hard: solid. 5. Clot: Character {soft: floculent. {ragged and broken up by gas {bubbles. (a) Coagulum undissolved. (b) Coagulum finally peptonised, completely: incompletely. Resulting solution, clear: turbid. {Abundant. {Scanty. 6. Whey: {Clear. {Turbid. {Coagulated by boiling, or not. ~BY MICROSCOPICAL METHODS.~ As a council of perfection preparations must be made from pure cultivations 4, 6, 8, 12, 18, and 24 hours; and subsequently at intervals of, say, twenty-four hours, during the entire period they are under observation, and examined-- (A) ~Living.--1.~ In ~hanging drop~, to determine _motility_ or _non-motility_.