The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.Eyre, J. W. H. (John William Henry)
Science
The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
Eyre, J. W. H. (John William Henry)
Bacteriology -- Technique
Repand: Like the border of an open umbrella (Fig. 148, c).
Erose: As if gnawed, irregularly toothed (Fig. 148, d).
[Illustration: FIG. 148.--Edges of colonies: a, Entire; b, undulate;
c, repand; d, erose.]
Lobate.
Lobulate: Minutely lobate (Fig. 149, e).
Auriculate: With ear-like lobes (Fig. 149, f).
Lacerate: Irregularly cleft, as if torn (Fig. 149, g).
Fimbriate: Fringed (Fig. 149, h).
Ciliate: Hair-like extensions, radiately placed (Fig. 149, j).
Tufted.
Filamentous: As already defined.
Curled: As already defined.
[Illustration: FIG. 149.--Edges of colonies: e, Lobar-lobulate; f,
auriculate; g, lacerate; h, fimbriate; i, ciliate.]
(F) ~Optical Characters~ (after Shuttleworth).--
1. _General Characters_:
Transparent: Transmitting light.
Vitreous: Transparent and colourless.
Oleaginous: Transparent and yellow; olive to linseed-oil coloured.
Resinous: Transparent and brown, varnish or resin-coloured.
Translucent: Faintly transparent.
Porcelaneous: Translucent and white.
Opalescent: Translucent; greyish-white by reflected light.
Nacreous: Translucent, greyish-white, with pearly lustre.
Sebaceous: Translucent, yellowish or greyish-white.
Butyrous: Translucent and yellow.
Ceraceous: Translucent and wax-coloured.
Opaque.
Cretaceous: Opaque and white, chalky.
Dull: Without lustre.
Glistening: Shining.
Fluorescent.
Iridescent.
2. _Chromogenicity_:
Colour of pigment.
Pigment restricted to colonies.
Pigment restricted to medium surrounding colonies.
Pigment present in colonies and in medium.
~Streak or Smear Cultures.~--
_Gelatine and Agar._--Note general points as indicated under plate
cultivations.
_Inspissated Blood-serum._--Note the presence or absence of liquefaction
of the medium. (The presence of condensation water at the bottom of the
tube must not be confounded with liquefaction of the medium.)
_All Oblique Tube Cultures._--
1. Colonies Discrete: Size, shape, etc., as for plate cultivations
(_vide_ page 261).
2. Colonies Confluent: Surface elevation and character of edge, as for
plate cultivations (_vide_ page 263).
Chromogenicity: As for plate cultures.
~Gelatine Stab Cultures.~--
(A) _Surface Growth._--As for individual colonies in plate cultures
(_vide_ page 261).
[Illustration: FIG. 150.--Stab cultivations--types of growth: a,
Filiform; b, beaded; c, echinate; d, villous; e, arborescent.]
(B) _Line of Puncture._--
Filiform: Uniform growth, without special characters (Fig. 150, a).
Nodose: Consisting of closely aggregated colonies.
Beaded: Consisting of loosely placed or disjointed colonies (Fig. 150,
b).
Papillate: Beset with papillate extensions.
Echinate: Beset with acicular extensions (Fig. 150, c).
Villous: Beset with short, undivided, hair-like extensions (Fig. 150,
d).
Plumose: A delicate feathery growth.
[Illustration: FIG. 151.--Stab cultivations--types of growth: f,
Crateriform; g, saccate; h, infundibuliform; j, napiform; k,
fusiform; l, stratiform.]
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