The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.Eyre, J. W. H. (John William Henry)
Science
The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
Eyre, J. W. H. (John William Henry)
Bacteriology -- Technique
Some forms of filter candle are made with the open end contracted into a
delivery nozzle, which is glazed. In this case the apparatus is fitted
up in a slightly different manner; the fluid to be filtered is contained
in an open cylinder into which the candle is plunged, while its delivery
nozzle is connected with the filter flask by means of a piece of
flexible pressure tubing (previously sterilised by boiling), as in
figure 39.
IV. THE MICROSCOPE.
The essentials of a microscope for bacteriological work may be briefly
summed up as follows:
[Illustration: FIG. 40.--Microscope stand.]
The instrument, of the monocular type, must be of good workmanship and
well finished, rigid, firm, and free from vibration, not only when
upright, but also when inclined to an angle or in the horizontal
position. The various joints and movements must work smoothly and
precisely, equally free from the defects of "loss of time" and
"slipping." All screws, etc., should conform to the Royal Microscopical
Society's standard. It must also be provided with good lenses and a
sufficiently large stage. The details of its component parts, to which
attention must be specially directed, are as follows:
[Illustration: FIG. 41.--Foot, three types.]
~1. The Base or Foot~ (Fig. 40, a).--Two elementary forms--the tripod
(Fig. 41, a) and the vertical column set into a plate known as the
"horse-shoe" (Fig. 41, b)--serve as the patterns for countless
modifications in shape and size of this portion of the stand. The chief
desiderata--stability and ease of manipulation--are attained in the
first by means of the "spread" of the three feet, which are usually shod
with cork; in the second, by the dead weight of the foot-plate. The
tripod is mechanically the more correct form, and for practical use is
much to be preferred. Its chief rival, the Jackson foot (Fig. 41, c),
is based upon the same principle, and on the score of appearance has
much to recommend it.
~2.~ The ~body tube~ (Fig. 40, b) may be either that known as the "long"
or "English" (length 250 mm.), or the "short" or "Continental" (length
160 mm.). Neither length appears to possess any material advantage over
the other, but it is absolutely necessary to secure objectives which
have been manufactured for the particular tube length chosen. In the
high-class microscope of the present day the body tube is usually
shorter than the Continental, but is provided with a draw tube which,
when fully extended, gives a tube length greater than the English, thus
permitting the use of either form of objective.
[Illustration: FIG. 42.--Coarse adjustment.]
[Illustration: FIG. 43.--Fine adjustment.]
For practical purposes the tube length = distance from the
end of the nosepiece to the eyeglass of the ocular. This is
the measurement referred to in speaking of "long" or "short"
tube.
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