Distilled water 100 c.c. Add anilin oil drop by drop (shaking well after the addition of each drop) until the solution is opaque. Filter until clear. and add Absolute alcohol 10 c.c. Saturated alcoholic solution gentian violet 11 c.c. Filter. NOTE.--This solution will not keep longer than 14 days. ~Thionine Blue (or Lauth's Violet).~-- 9. _Carbolic Thionine Blue_ (Nicollé). Weigh out Thionine blue 1.0 gramme Carbolic acid 2.5 grammes and dissolve in Distilled water 100.0 c.c. Filter. Before use dilute with equal quantity of distilled water and again filter. ~Fuchsin (Basic).~-- 10. _Saturated Aqueous Solution._ Weigh out Basic fuchsin 1.5 grammes and proceed as in preparing the corresponding solution of methylene-blue (_q. v._). 11. _Saturated Alcoholic Solution._ Weigh out Basic fuchsin 3.5 grammes and proceed as in preparing the corresponding solution of methylene-blue. 12. _Carbolic Fuchsin_ (Ziehl). Weigh out Basic fuchsin 1.0 gramme Carbolic acid 5.0 grammes dissolve in Distilled water 100.0 c.c. and add Absolute alcohol 10.0 c.c. Filter. CONTRAST STAINS. ~Eosin.~--There are several commercial varieties of eosin, which, from the bacteriological point of view, possess very different values. Gruebler lists four varieties, of which two only are useful for bacteriological work: Eosin, aqueous yellowish. Eosin, aqueous bluish. 13. _Eosin Aqueous Solution_ (Yellowish or Bluish Shade), 1 per cent. Weigh out Eosin, aqueous 1.0 gramme dissolve in Distilled water 100.0 c.c. and add Absolute alcohol 5.0 c.c. Filter. 14. _Eosin Alcoholic Solution_, 0.5 per cent. Weigh out Eosin, alcoholic 0.5 gramme and dissolve in Alcohol (70 per cent.) 100.0 c.c. Filter. ~Safranine.~-- 15. _Aqueous Solution._ Weigh out. Safranine 0.5 gramme and dissolve in Distilled water 100.0 c.c. Filter. ~Neutral Red.~-- 16. _Aqueous Solution._ Weigh out Neutral red 1.0 gramme and dissolve in Distilled water 100.0 c.c. Filter. ~Vesuvin (or Bismarck Brown).~-- 17. _Saturated Aqueous Solution._ Weigh out Vesuvin 0.5 gramme and dissolve in Distilled water 100.0 c.c. Filter. TISSUE STAINS. ~Aniline Gentian Violet~ (For Weigert's Fibrin Stain).-- Weigh out Gentian violet 1.0 gramme and dissolve in Absolute alcohol 15.0 c.c. Distilled water 80.0 c.c. then add Aniline oil 3.0 c.c. Shake well and filter before use. ~Hæmatoxylin~ (Ehrlich).-- 1. Weigh out Hæmatoxylin 2.0 grammes and dissolve in Absolute alcohol 100.0 c.c. 2. Weigh out Ammonium alum 2.0 grammes and dissolve in Distilled water 100.0 c.c.