=How humus and the subsoil are mingled.= The mingling of humus and the subsoil is brought about by several means. The roots of plants penetrate the waste, and when they die leave their decaying substance to fertilize it. Leaves and stems falling on the surface are turned under by several agents. Earthworms and other animals whose home is in the waste drag them into their burrows either for food or to line their nests. Trees overthrown by the wind, roots and all, turn over the soil and subsoil and mingle them together. Bacteria also work in the waste and contribute to its enrichment. The animals living in the mantle do much in other ways toward the making of soil. They bring the coarser fragments from beneath to the surface, where the waste weathers more rapidly. Their burrows allow air and water to penetrate the waste more freely and to affect it to greater depths. =Ants.= In the tropics the mantle of waste is worked over chiefly by ants. They excavate underground galleries and chambers, extending sometimes as much as fourteen feet below the surface, and build mounds which may reach as high above it. In some parts of Paraguay and southern Brazil these mounds, like gigantic potato hills, cover tracts of considerable area. In search for its food--the dead wood of trees--the so-called white ant constructs runways of earth about the size of gas pipes, reaching from the base of the tree to the topmost branches. On the plateaus of central Africa explorers have walked for miles through forests every tree of which was plastered with these galleries of mud. Each grain of earth used in their construction is moistened and cemented by slime as it is laid in place by the ant, and is thus acted on by organic chemical agents. Sooner or later these galleries are beaten down by heavy rains, and their fertilizing substances are scattered widely by the winds. =Earthworms.= In temperate regions the waste is worked over largely by earthworms. In making their burrows worms swallow earth in order to extract from it any nutritive organic matter which it may contain. They treat it with their digestive acids, grind it in their stony gizzards, and void it in castings on the surface of the ground. It was estimated by Darwin that in many parts of England each year, on every acre, more than ten tons of earth pass through the bodies of earthworms and are brought to the surface, and that every few years the entire soil layer is thus worked over by them. In all these ways the waste is made fine and stirred and enriched. Grain by grain the subsoil with its fresh mineral ingredients is brought to the surface, and the rich organic matter which plants and animals have taken from the atmosphere is plowed under. Thus Nature plows and harrows on "the great world's farm" to make ready and ever to renew a soil fit for the endless succession of her crops.