In 1848, Lieutenant Spratt read a paper at the meeting of the British Association at Swansea, on the influence of temperature upon the distribution of the fauna in the Ægean seas, and at the close of this paper we find the following passage, confirming in a remarkable way the work of previous investigators in the same field. He says: ‘The greatest depth at which I have procured animal life is 390 fathoms, but I believe that it exists much lower, although the general character of the Ægean is to limit it to 300 fathoms; but as in the deserts we have an oasis, so in the great depths of 300, 400, and perhaps 500 fathoms we may have an oasis of animal life amidst the barren fields of yellow clay dependent upon favourable and perhaps accidental conditions, such as the growth of nullipores, thus producing spots favourable for the existence and growth of animal life.’ The next important discovery was that of the now famous Globigerina mud by Lieutenants Craven and Maffit, of the American Coast survey, in 1853, by the help of the sounding machine invented by Brooke. This was reported upon by Professor Bailey. Further light was thrown upon the deep-sea fauna by Dr. Wallich in 1860, on board H.M.S. ‘Bulldog’, by the collection of thirteen star-fish living at a depth of 1,260 fathoms. Previous to this Torell, during two excursions to the Northern seas, had proved the existence of an extensive marine fauna in 300 fathoms, and had brought up with the ‘Bulldog’ machine many forms of marine invertebrates from depths of over 1,000 fathoms; but it was not until 1863, when Professor Lovén read a report upon Torell’s collections, that these interesting and valuable investigations became known to naturalists. Nor must mention be omitted of the remarkable investigations of Sars and his son, the pioneers of deep-sea zoology on the coasts of Norway, who, by laborious work commenced in 1849, failed altogether to find any region in the deep water where animal life was non-existent, and indeed were the first to predict an extensive abysmal fauna all over the floor of the great oceans. One of the many remarkable discoveries made by Sars was _Rhizocrinus_, a stalked Crinoid. Ever since that time the Norwegians and the Swedes have been most energetic in their investigations, and the publications of the results of the Norske Nord-havns expeditions are regarded by all naturalists as among the most valuable contributions to our knowledge of the deep-sea fauna. Notwithstanding the previous discovery of many animals that undoubtedly came from the abysmal depths of the ocean, there were still some persons who found a difficulty in believing that animal life could possibly exist under the enormous pressure of these great depths. It was considered to be more probable that these animals were caught by the dredge or sounding lines in their ascent or descent; and that they were merely the representatives of a floating fauna living a few fathoms below the surface.