Cell Structures--Cell Wall--Protoplasm--Plasmolysis --Plasmoptysis--Nucleus--Vacuoles--Capsules--Metachromatic Granules--Flagella--Spores 41 CHAPTER III. Cell Forms--Coccus--Bacillus--Spirillum--Involution Forms 52 CHAPTER IV. Cell Groupings 55 CHAPTER V. Classification--Migula’s--Society of American Bacteriologists’--Key to the Latter 59 PART II. PHYSIOLOGY. CHAPTER VI. Occurrence--General Conditions for Growth--Moisture--Temperature--Light--Oxygen-- Osmotic Pressure--Electricity--X-rays and Radium Emanations--Pressure--Mechanical Vibration 71 CHAPTER VII. Chemical Environment--Reaction of Medium--Chemical Composition 81 CHAPTER VIII. Chemical Environment (Continued)--General Food Relationships--Metabolism of Elements 86 CHAPTER IX. Physiological Activities--Fermentation of Carbohydrates--Splitting of Fats 93 CHAPTER X. Physiological Activities (Continued)--Putrefaction of Proteins--Cycles of Nitrogen, Carbon, Sulphur, Phosphorus 102 CHAPTER XI. Physiological Activities (Continued)--Production of Acids, Gases, Esters, Alcohols, Aldehydes, Aromatic Compounds--Phosphorescence--Chromogenesis--Reduction-- Oxidation--Production of Heat--Absorption of Free Nitrogen--Nitrogen Nutrition of Green Plants 110 CHAPTER XII. Physiological Activities (Continued)--Production of Enzymes--Discussion on Enzymes--Toxins--Causation of Disease 121 CHAPTER XIII. Disinfection--Sterilization--Disinfectants--Physical Agents--Pasteurization 130 CHAPTER XIV. Disinfection and Sterilization (Continued)--Chemical Agents--Anilin Dyes 156 CHAPTER XV. Disinfection and Sterilization (Continued)--Choice of Agent--Standardization of Disinfectants--Phenol Coefficient--Practical Sterilization and Disinfection 164 PART III. THE STUDY OF BACTERIA. CHAPTER XVI. Culture Media--Broth, Milk, Gelatin, Agar, Potatoes, Blood Serum--Standardization of Media--H-ion Concentration Method--Synthetic Media 171 CHAPTER XVII. Methods of Using Culture Media--Culture Tubes--Plates-- Anaërobic Cultures--Vignal Tubes--Fermentation Tubes-- Deep Culture Tubes--Novy Jars--Inoculation of Culture Media 184 CHAPTER XVIII. Isolation of Bacteria in Pure Culture--Dilution --Plating--Streaking--Barber Apparatus--Aids in Isolation--Heat--Selective Antiseptics--Selective Food---Indicators--Animal Inoculation 194 CHAPTER XIX.