The Fundamentals of BacteriologyMorrey, Charles Bradfield
Science
The Fundamentals of Bacteriology
Morrey, Charles Bradfield
Bacteriology
As to the form in which the last six elements in the table exist in
the cell, little is known. The sulphur and phosphorus are essential
constituents of various proteins. The high percentage of phosphorus
points to nuclein compounds as its probable source.
The carbon and nitrogen, together with most of the hydrogen and oxygen
not united as water, make up the great variety of organic compounds
which compose the main substances in the bacterial cell.
It has already been stated that the essential structures in the
bacterial cell are cell wall and protoplasm, including the nuclein.
These differ markedly in chemical composition. It is well known that
the cell walls of green plants consist largely of cellulose and closely
related substances.[7] _True cellulose_ has been recognized in but
very few bacteria. (_Sarcina ventriculi_, Migula; _Mycobacterium
tuberculosis_, Hammerschlag, Dreyfuss, Nishimura; _Bacillus
subtilis_, Dreyfuss; _Acetobacter xylinum_, Brown; _Acetobacter
acidi oxalici_, Banning; and a few others.) It is certainly not an
important constituent of the cell wall in many. On the other hand,
_hemicellulose_ and _gum-like_ substances have been identified in
numerous organisms of this class as important constituents of the cell
wall and of the capsule which is probably an outgrowth from the latter.
Practically always associated with these substances are compounds
containing nitrogen. One of these has been certainly identified as
_chitin_ or a closely similar substance. Chitin is the nitrogenous
substance which enters largely into the composition of the hard parts
of insects, spiders and crustaceans. It is an interesting fact to find
this substance characteristic of these animals in bacteria, as well as
other fungi.
Though it is extremely difficult to separate the cell wall of bacteria
from the cell contents, in the light of our present knowledge it can be
stated that the cell walls are composed of a carbohydrate body closely
related to cellulose, though not true cellulose, probably in close
combination with chitin.
Of the organic constituents of the cell contents the most abundant are
various proteins which ordinarily make up about one-half of the dry
weight of the entire cell. The “Mycoproteid” of Nencki, 1879, and other
earlier workers is deserving of little more than historical interest,
since these substances were certainly very impure and probably
consisted of mixtures of several “proteins” in the more recent sense.
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