The Fundamentals of BacteriologyMorrey, Charles Bradfield
Science
The Fundamentals of Bacteriology
Morrey, Charles Bradfield
Bacteriology
_Asepsis_ is a term which is restricted almost entirely to surgical
operations and implies the taking of such precautions that foreign
organisms are _kept out_ of the field of operation. Such an operation
is an _aseptic_ one, or performed _aseptically_.
A “deodorant or deodorizer” is used to destroy or remove an odor and
does not necessarily have either antiseptic or disinfectant properties.
The agents which are used for the above-described processes may be
conveniently divided into _physical agents_ and _chemical agents_.
PHYSICAL AGENTS.
=1. Drying.=--This is doubtless the oldest method for _preventing the
growth_ of organisms, and the one which is used on the greatest amount
of material at the present time. A very large percentage of commercial
products is preserved and transported intact because the substances
are kept free from moisture. In the laboratory many materials which
are used as food for bacteria (see Chapter XVI) “keep” because they
are dry. Nevertheless, drying should be considered as an _antiseptic_
rather than as a _disinfectant_ process. While it is true that the
_complete_ removal of water would result in the death of all organisms
this necessitates a high temperature, in itself destructive, and does
not occur in practice. Further, though many pathogenic bacteria are
killed by drying, many more, including the spore formers, are not.
Hence drying alone is not a practical method of _disinfecting_.
[Illustration: FIG. 77.--A small laboratory hot-air sterilizer.]
=2. Heat.=--The use of heat in some form is one of the very best
means for destroying bacteria. It may be made use of by combustion,
or burning, as direct exposure to the open flame, as dry heat (hot
air), or as moist heat (boiling water or steam). Very frequently in
veterinary practice, especially in the country, occasionally under
other conditions, the infected material is best burned. This method is
thoroughly effective and frequently the cheapest in the end. Wherever
there are no valid objections it should be used. Exposure to the open
flame is largely a laboratory procedure to sterilize small metallic
instruments and even small pieces of glassware. It is an excellent
procedure in postmortem examinations to burn off the surface of the
body or of an organ when it is desired to obtain bacteria from the
interior free from contamination with surface organisms.
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