The Fundamentals of BacteriologyMorrey, Charles Bradfield
Science
The Fundamentals of Bacteriology
Morrey, Charles Bradfield
Bacteriology
=Soap.=--Experimenters have obtained many conflicting results with
soaps when tested on different organisms, as is to be expected from
the great variations in this article. Miss Vera McCoy in the author’s
laboratory carried out experiments with nine commercial soaps--Ivory,
Naphtha, Packer’s Tar, Grandpa’s Tar, Balsam Peru, A. D. S. Carbolic,
German Green, Dutch Cleanser, Sapolio--and obtained abundant growth
from spores of _Bacillus anthracis_, from _Bacterium coli_ and from
_Staphylococcus pyogenes aureus_ in all cases even when the organisms
had been exposed twenty-four hours in 5 per cent. solutions. From
these results and from the wide variations reported in the literature
it is clear that _soap solutions alone cannot be depended on_ as
disinfectants. Medicated soaps do not appear to offer any advantages in
this respect. The amount of the disinfectant which goes into solution
when the soap is dissolved is too small to have any effect.
=Formaldehyde.=--Formaldehyde (HCHO) is perhaps the most largely used
chemical disinfectant at the present time. The substance is a gas
but occurs most commonly in commerce as a watery solution containing
approximately 40 per cent. of the gas. This solution is variously known
as formalin, formol, and formaldehyde solution. The first two names
are patented and the substance under these names usually costs more.
It is used in the gaseous form for disinfecting closed spaces of all
kinds to the exclusion of most other means today. A great many types
of formalin generators have been devised. The gas has little power of
penetration and all material to be reached should be exposed as much as
possible. The dry gas is almost ineffective, so that the objects must
be moistened or vapor generated along with the gas. A common method
in use is to avoid expensive generators by pouring the formaldehyde
solution on permanganate of potash crystals placed in a vessel removed
from inflammable objects on account of the heat developed which
occasionally sets the gas on fire. The formalin is used in amounts
varying from 20 to 32 ounces to 8-1/2 to 13 ounces of permanganate to
each 1000 cubic feet of space. This method is expensive since one pint
(16 ounces) of formalin is sufficient for each 1000 cubic feet, and
since the permanganate is an added expense. Dr. Dixon, Commissioner of
Health of Pennsylvania, recommends the following mixture to replace the
permanganate, claiming that it works more rapidly and is less expensive
and just as efficient:
1. Sodium bichromate, ten ounces.
2. Saturated solution of formaldehyde, sixteen ounces.
3. Common sulphuric acid, one and a half ounces.
Two and three are mixed together and when cool are poured on the
bichromate which is placed in an earthenware jar of a volume about ten
times the quantity of fluid used. The quantities given are for each
1000 cubic feet of space.
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