The next year (1860) Garibaldi, the lion-like fighter, the enthusiastic lover of Italy, gathering round him a thousand followers, made an unexpected attack on Sicily, which was held by the tyrant King of Naples. With his celebrated "Thousand" he won two remarkable victories. The Sicilians joined him; the Neapolitans were driven from the island. Not giving them time to recover, Garibaldi followed to the mainland, defeated them again, and was master of all Southern Italy. Meanwhile Victor Emmanuel, marching his troops southward, seized what was left of the States of the Church. The two conquerors met midway in Italy, and Garibaldi, grasping his sovereign by the hand, saluted him as King at last of a united Italy. Only Rome and Venice remained outside the pale, Rome protected by being in actual possession of the Pope, and, since France was still Catholic, guarded by French troops from the eager Italians. The year 1860 had been second only to 1848 in its importance in changing the outlines of modern Europe. [Footnote: See _The Kingdom of Italy Established_.] Another change, immeasurably vast and still unmeasured in its consequences, may be dated from 1859, when Charles Darwin gave to the world his book, the _Origin of Species_. In this he proclaimed the doctrine of the evolution of all the more complicated forms of life from simpler forms. The idea, at first resolutely combated on religious grounds, has gradually received more or less acceptance into the entire religious fabric, even as were the discoveries of Galileo. [Footnote: See _Darwin Publishes His Origin of Species_.] DISUNION IN AMERICA Yet each and all of these events, important as they were, grew little in men's minds as the year 1860 drew to its close and revealed in America the coming of a mightier quarrel. The slavery question, once supposed to have been settled by the Missouri Compromise, had proved itself incapable of such settlement. The forward march of democracy had in fact made slavery an anachronism, outgrown and impossible. Even the Emperor of Russia saw that, and in 1861 liberated all the serfs within his territories. [Footnote: See _Emancipation of Russian Serfs_.] In the United States alone among the great Powers of the world, did slavery persist. In 1854 a new political party, calling itself the Republican, was formed, having for its main principle opposition to the extension of slavery into the Territories. [Footnote: See _The Rise of the Republican Party_.] Other issues might and did complicate the central question, but it was the slavery issue that inflamed men's minds, made Kansas a "battle-ground" between settlers from North and South, and sent John Brown upon his reckless raid. Watching the increasing success of the Republicans, Southern leaders began to reassert the doctrine of the right of secession. They said openly that if a Republican president were elected they would leave the Union.