Spain regretted the death of Don Carlos, as the king had no other son. By his third wife, Elizabeth or Isabella of France, he had only had two daughters, and that virtuous princess died of a miscarriage in the same year, 1568. This misfortune (and the bad opinion conceived by all Europe of Philip II., who was considered as a cruel and hypocritical prince) occasioned the imputation of having caused the queen's death. He was first accused of it by the Prince of Orange, and afterwards by many other persons. France had proofs of the contrary, since Charles IX. sent an ambassador extraordinary to Madrid, with compliments of condolence to the king, who was really inconsolable for the loss of his expected heir. Juan Lopez del Hoyo, in 1569, published a faithful account of the illness and death of the queen; and some circumstances which he mentions seem incompatible with the use of poison, which is said to have occasioned her death. It is evident that the Prince of Orange suffered himself to be misled by hatred and revenge. The reality of a crime cannot be believed when neither the end nor motives for it can be perceived, and Philip was certainly interested in the queen's life. Some writers, after having supposed that the crime was committed, have endeavoured to discover the cause, and some romance-writers have thought that they discovered it in the pretended intrigue with Don Carlos. Supposing it to be true, there are historical proofs that it could not have commenced till after his return from Alcala, and at that time he ardently wished to marry his cousin, Anne of Austria. This princess became the fourth wife of Philip, and the mother of his successor, Philip III. Philip II., wishing to commemorate the justice of his conduct towards his son, ordered that the writings of the trial, with the original, and translation from the Catalonian tongue of that of Don Charles, Prince of Biana, should be collected and preserved. Don Francis de Mora, Marquis de Castel Rodrigo, who became the king's confidant after the death of Rui Gomez de Sylva, in 1592, deposited these writings in a green coffer, which the king afterwards sent shut, and without a key, to the royal archives of Simancas, where it is still, if it has not been carried away by the order of the French government, as it has been reported in Spain. CHAPTER XXXII. TRIAL OF THE ARCHBISHOP OF TOLEDO.