In the meantime, the testicular biopsies, in their fluid-filled
bottles, were on their way to Tissue Path., to join those that Smith
and his residents were already preparing from the convalescent ferrets.
Speaking into Dictape machines, the junior residents described and
numbered the specimens while deft-fingered girl technicians wrapped
them in little packets and put those in tiny perforated boxes. They
dropped the boxes into beakers filled with fixative which they then set
up on the Technicon machines. The dials were set, the clock ticked,
and hour by hour, as the timer clicked into the grooves of the wheel,
the arms of the Technicon lifted, dangling their clusters of dripping
boxes, turned like soldiers on parade, and dropped them again into
the next beaker. On they went through the fixative that preserved the
cells as they had been in life, the alcohols that slowly and carefully
removed the water, the xylol that replaced the alcohol and, finally,
the hardened shreds of tissue lay in melted paraffin, ready for the
cutting.
But first they had to be embedded in paper boats full of melted wax
which, when it hardened, held them securely. Then, in millionths of a
metre, the incredibly fine edge of the microtome sliced off a ribbon of
tissue, as a bacon slicer cuts pork. The technician laid the ribbons
on a bowl of warm water, separated off each individual slice with her
needle and guided it on to a prepared glass slide which was then laid
aside to dry. That was not all. Now the process had to be reversed, the
paraffin removed with xylol, the xylol with alcohol, the alcohol with
water, before the pale white dots of tissue could be stained. There was
no way of hurrying the process. Chemicals need time to react, and time
they took, regardless of our impatience. At last the blue color of the
Hematoxylin and the red of the eosin had been added in their turn and
taken up by the tissues; the protective balsam and the slip cover had
been placed over the sections; the slides had hardened enough to be put
under the microscope.
With mounting excitement, Smith and his senior residents racked down
the binocular microscopes to focus on the minute blue and red dots
that lay beneath. Silently they looked, moving the slides jerkily but
accurately with their fingers to view all the sections. Still silent,
they swapped slides to check and re-check their findings. At last
Smith straightened up and removed his spectacles. He rubbed his eyes
wearily. He looked along the table at the three young men who had
worked with him.
"Any doubts about this?" he said.
Three heads shook slightly. There was nothing to say. They were too
tired for casual chatter. He pushed the Intercom switch.
"Dr. Hallam. Smith calling."
The sound came into the living room as we sat at midnight coffee. The
rasping voice jarred us out of the apathy of exhaustion.
"This is Hallam."
Public-domain text, read in full here on John Shaqi.
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