The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
The same may be said of the intermediate
colours of the other pencils. The eye-glass thus constructed not only
brings together the images _b′ b′_, _r′ r′_, but it likewise has the
most important effect of rendering them flatter, and assisting in the
correction of chromatic and spherical aberration.
[Illustration: Fig. 100.--Huyghenian Eye-piece.]
[Illustration: Fig. 101.--Ramsden’s Eye-piece.]
The later form of the Huyghenian eye-piece is that of the late Sir
George Airy, the field-glass of which is a meniscus with the convex
side turned towards the objective, and the eye-lens a crossed convex
with its flatter side towards the eye. Another negative eye-piece is
that known as the _Kellner_, or orthoscopic eye-piece. It consists
of a bi-convex field-glass and an achromatic doublet eye-lens. This
magnifies ten times, but it in no way compares with the Huyghenian in
value. Neither does it afford the same flatness of field.
The _Ramsden_, or positive eye-piece, is chiefly employed as a
micrometer eye-piece for the measurement of the values of magnified
images. The construction of this eye-piece is shown in Fig. 101, a
divided scale being cut on a strip of glass in 1/100ths of an inch,
every fifth of which is cut longer than the rest to facilitate the
reading of the markings, and at the same time that of the image of the
object, both being distinctly seen together, as in the accompanying
reduced micro-photograph of blood corpuscles, Fig. 102.
The value of such measurements in reference to the real object, when
once obtained; is constant for the same objective. It becomes apparent,
then, that the value of the divisions seen in the eye-piece micrometer
must be found with all the objectives used, and carefully tabulated.
It was Mr. Lister who first proposed to place on the stage of the
microscope a divided scale of a certain value. Viewing the scale as a
microscopic object, he observed how many of the divisions on the scale
attached to the eye-piece corresponded with one or more of a magnified
image. If, for instance, ten of those in the eye-piece correspond with
one of those in the image, and if the divisions are known to be equal,
then the image is ten times larger than the object, and the dimensions
of the object ten times less than that indicated by the micrometer.
If the divisions on the micrometer and on the magnified scale are not
equal, it becomes a mere rule-of-three sum; but in general this trouble
is taken by the maker of the instrument, who furnishes a table showing
the value of each division of the micrometer for every object-glass
with which it will be employed.
[Illustration: Fig. 102.--Blood Corpuscles and Micrometer, magnified
1·3500.]
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