The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
In the microscope the _parabolic reflector_ fits into the cylindrical
fitting under the stage, and the adjustment of its focus upon the
object is made by giving it a spiral motion when fitted in--that is,
carefully pushing it up or down at the same time that it is turned
round by the milled edge B B. It must then be focussed by the rack
and pinion motion. As the rays of light must be parallel when they
enter it, a _flat mirror_, which in this case should be added to the
instrument, is generally used; daylight will then require only direct
reflection, but the rays from an artificial source will have to be made
parallel by placing a side condenser between the light and the mirror,
about 1-3/4 inch from the former and 4-1/2 inches from the latter.
Nearly the whole surface of the mirror should be equally illuminated;
this may be tested by temporarily placing upon it a card or piece
of white paper. Parallel rays can also be obtained from the concave
mirror, if the light is placed about 2-1/2 inches from it. Dark-ground
illumination is not suitable for very transparent objects--that is,
unless there is a considerable difference in their index of refraction,
or they are pervaded by air-cells.
[Illustration: Fig. 145.--Parabolic Reflector.]
One very remarkable example of this may be seen in the tracheal system
of insects. If any of the transparent larvæ of the various kinds of
gnat be mounted in gelatine and glycerine jelly, slightly warmed
but not enough to kill the insect outright, about the third day the
fluids circulating in the body will be absorbed and replaced by air.
Illuminated by the parabolic condenser, and viewed with a binocular
microscope, and a low power, the gnat-larva becomes a superb object.
The body of the insect is but faintly visible, and in its place is
displayed a marvellous tracheal skeleton, with the tubes standing out
in perspective, shining brilliantly, like a structure of burnished
silver. Unfortunately, such objects are not permanent, for when the
whole of the water dries up, the tracheal tubes either collapse or
become refilled with fluid.
As to the blackness of field, and luminosity of the object, this
depends upon excess of light from the paraboloid received beyond the
angle of aperture of the object-glass. It is found in practice that
more and more of the inner annulus of rays from the paraboloid has
to be stopped off, until at last, with high-angled objectives, it is
scarcely possible to obtain a black field.
The light, on the whole, most suitable for this method of illumination
is lamp, the rays of which should in all cases be rendered more
parallel by means of a large plano-convex lens, or condenser.
[Illustration: Fig. 146.--Wenham’s Hemispherical Lens.]
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