The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
_Wenham’s Immersion Condenser._--Mr. Wenham, in the year 1856,
described various forms of oblique illuminators, one of which was an
immersion; a simple right-angled prism, connected by a fluid medium of
oil of cloves. This, however, was abandoned for a nearly hemispherical
lens connected with the slide, and although an improvement, did not
touch the point of excellence Mr. Wenham was looking for. Ultimately
he adopted a semi-circular disc of glass of the exact form and size
represented in the drawing, Fig. 146, having a quarter-inch radius,
with a well-polished rounded edge, the sides being grasped by a simple
kind of open clip attached to the sub-stage, the fluid medium used
for connecting the upper surface with the slide being either water,
glycerine, or oil; an increase of oblique illumination being obtained
by swinging the ordinary mirror sideways. By means of an illuminator of
the kind difficult objects mounted in balsam are resolved. This simple
piece of glass collects and concentrates light in a marvellous manner,
and is by no means a bad substitute for some of the more costly forms
of achromatic condenser. It can be used either in fluid contact with
the slide, or dry, as an ordinary condenser.
Mr. Wenham subsequently contrived a small truncated glass paraboloid,
for use in fluid contact with the slide; water, glycerine, oil, or
other substance being employed as a contact medium. The rays of light
in this illuminator, being internally reflected from a convex surface
of glass, impinge obliquely on the under surface of the slide, and
are transmitted by the fluid uniting medium, and internally reflected
from the upper surface of the cover-glass to the objective. To use the
reflex illuminator efficiently it must be racked up to a level with the
stage. The centre of rotation is then set true by a dot on the fitting,
seen with a low power, a drop of water is then placed on the top, and
upon this the slide is laid. Minute objects _on the slide_ must be
found either by the aid of a low power, by their greater brilliancy, or
by rotating the illuminator; the effect on the podura scale is superb,
the whole scale appearing dotted with bright blue spots in a zig-zag
direction. Objects for this illuminator should be especially selected
and mounted.
[Illustration: Fig. 147.--The Amici Prism.]
The Amici Prism, originally designed for oblique illumination, consists
of a flattened triangular glass prism, the two narrower sides of
which are slightly convex, while the third or broadest side forms the
reflecting surface. When properly used, it is capable of transmitting
a very oblique pencil of light. The prism is either mounted, as in
Fig. 147, for slipping into the fitting of the sub-stage, or on an
independent stand, as arranged for Powell’s microscope, page 85, Fig.
56.
Method of Employing the Achromatic Condenser to the Greatest Advantage.
Public-domain text, read in full here on John Shaqi.
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