The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
[Illustration: Fig. 165.--Maltwood’s Finder.]
Dr. Pantacsek’s finder appears to have some advantage over Maltwood’s,
but it cannot be used with the same facility, and therefore will not
displace an old favourite. _The Amyot_ finder I have long had in use;
it is efficient and inexpensive--can indeed, if misplaced or lost, be
replaced by the aid of the square and compasses.
[Illustration: Fig. 166.--Amyot’s Object Finder.]
_The Okeden_ finder consists of two graduated scales, one _vertical_,
attached to the fixed stage-plate, the other _horizontal_, attached to
an arm carried by the intermediate plate; the first of these scales
enables the worker to “set” the vertically-sliding plate to any
determinate position in relation to the fixed plate, while the second
gives the power of setting the horizontally-sliding plate by that of
the intermediate.
_Micrometers._--It is of the utmost importance to have a means of
measuring with accuracy the objects, or part of objects, under
observation. The most efficient piece of apparatus for the purpose is
the micrometer eye-piece, the earlier form of which, Jackson’s, has
been described under the heading _Eye-pieces_ (p. 144). In the case of
micrometers, as in that of most other accessories, every optician has
his own adaptation and method of employing the same.
For the measurement of bacteria, a stage micrometer should be used with
a camera lucida. The stage micrometer consists of a slip of thin glass
ruled with a scale consisting of tenths and hundredths of a millimetre.
The image is projected on to a piece of paper placed on the table, and
the drawing made, and the object to be measured can be readily compared
with the scale.
[Illustration: Fig. 167.--The Ramsden Micrometer Eye-piece.]
In the Ramsden micrometer eye-piece, as previously explained, two fine
wires are stretched across the field of an eye-piece, one of which can
be moved by a micrometer screw. In the field there is also a scale with
teeth, and the interval between them corresponds to that of the threads
of the screw.
The circumference of the brass head is usually divided into one hundred
parts, and a screw with one hundred threads to the inch is used. The
bacterium to be measured is brought into a position in which an edge
appears to be in contact with the fixed wire, and the micrometer screw
is turned until the travelling wire appears to be in contact with
the other edge. The scale in the field and scale on the milled head,
together, give the number of complete turns of the screw and the value
of a fraction of a turn in separating the wires.
In the micrometer eye-piece constructed by Zeiss, the eye-piece with
a glass plate with crossed lines is carried across the field by means
of a micrometer screw. Each division on the edge of a drum corresponds
to ·01 mm. Complete revolutions of the drum are counted by means of a
figured scale in the visual field.
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