The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
So valuable a means of research has this process of analysis proved to
be, that the discoveries made by the spectroscope appear marvellous.
The spectroscope was first made known as a refined instrument for the
analysis of light by two Germans, a physicist and a chemist, Kirschoff
and Bunsen. In 1860, the latter succeeded in detecting and separating
two new alkaline bodies from all other bodies from the waters obtained
from the Durkeim springs, less than 0·0002 part of a milligramme of
which can be detected by spectrum analysis. It is to the labours
of Huggins, Norman Lockyer and others that we are indebted for the
wonderful discoveries made in astronomy; and chiefly so to Brewster,
Herschel, and Talbot, for showing that certain metals give off light of
a high degree of refrangibility; that distinct bands are situated at a
distance beyond the last visible violet ray ten times as great as the
length of the whole visible spectrum from red to violet.
With regard to the discoveries made in connection with physiological
research, we are indebted to F. Hoppe, who in 1862 first described
the absorption bands of human blood. His results were confirmed by
the investigations of Professor Sir George Gabriel Stokes, who, by
adding certain reducing agents to the blood, found that he could change
scarlet blood into purple--“purple cruorine”--and in this way the
place occupied by the absorption band in the spectrum could be made to
change. He reduced the hæmoglobin by robbing the blood of its oxygen.
Thus, by Stokes’ and other methods, we have since arrived at extremely
valuable results, and the explanation of the difference in colour
between arterial and venous blood; and it has also enabled us to show
wherein the breathing power of the red corpuscles resides, and further
explains phenomena which before his investigations were inexplicable.
[Illustration: Fig. 191.--Fräunhofer’s Spectrum Lines.]
The spectroscope seems likely to be of almost as great use in medicine
as it has already proved to be in solar and terrestrial chemistry, if
we may form an opinion from the large amount of literature which has
appeared on the subject. The inception of this magical instrument arose
on the instance of a discovery made by Dr. Wollaston in 1802, who, on
making a slit in the shutter of his room, instead of a round hole, the
spectrum of sunlight, instead of being composed of a number of coloured
discs, was now a band of pure colours, each colour being free from
admixture with the next to it. Moreover, he found that this colour band
was not continuous, as Newton described it, but interrupted here and
there by _fine black lines_.
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