The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
For most absorption-bands, particularly if faint, the prism should be
used in the first position, in which it gives the least dispersion;
when greater dispersion is required, so as to separate some
particular lines more widely, or to show the spectra of the metals,
or Fräunhofer’s lines in the solar spectrum, then the prism must be
used as in Fig. 193_a_. This answers well for liquids or transparent
objects, but it is, of course, not applicable to opaque objects.
To combine both purposes, some form of direct vision-prisms that maybe
applied to the body of the microscope is required. Fig. 194 represents
an arrangement of direct vision-prisms, invented by Herschel. The line
R R′ shows the path of a ray of light through the prisms, where it
would be seen that the emergent ray R′ is parallel and coincident with
the incident ray R.
[Illustration: Fig. 194.]
[Illustration: Fig. 194_a_.]
Another very compact combination is shown in Fig. 194_a_. Any number
of these prisms (P P P) may be used, according to the amount
of dispersion required. They are mounted in a similar way to a
Nicol’s prism, and are applied directly over the eye-piece of the
microscope. The slit S S is placed in the focus of the first glass
(F) if a negative, or below the second glass if a positive eye-piece
be employed. One edge of the slit is movable, and, in using the
instrument, the slit is first opened wide, so that a clear view of
the object is obtained. The part of the object of which the spectrum
is to be examined is then made to coincide with the fixed edge of the
slit, and the movable edge is screwed up, until a brilliant coloured
spectrum is produced. The absorption-bands will then be readily found
by slightly altering the focus. This contrivance answers perfectly
for opaque objects, without any preparation; and, when desirable, the
same prism can be placed below the stage, and a micrometer used in the
eye-piece of the microscope, thus avoiding a multiplicity of apparatus.
[Illustration: Fig. 195.--The Sorby-Browning Micro-spectroscopic
Eye-piece.]
A later and better form of instrument is the Sorby-Browning eye-piece
(Fig. 195), shown in section (Fig. 196) ready for inserting into the
body-tube of the microscope, the prism of which is contained in a
small tube, removable at pleasure. Below the prism is an achromatic
eye-piece, having an adjustable slit between the two lenses, the upper
lens being furnished with a screw motion to focus the slit. A side
slit, capable of adjustment, admits, when required, a second beam of
light from any object whose spectrum it is desired to compare with that
of the object placed on the stage of the microscope. This second beam
of light strikes against a very small prism, suitably placed inside the
apparatus, and is reflected up through the compound prism, forming a
spectrum in the same field with that obtained from the object on the
stage.
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