The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
The strongest light is reflected from the concave side of the mirror,
that from the flat side is more diffuse and less intense. Oblique light
can be obtained by turning the mirror on one side and then adjusting
it so as to illuminate the field from that position. All the necessary
mechanism of the microscope is easily and quickly learned. The
object-glasses or objectives are, as previously explained, designated
according to the focal distance of a single lens of the same magnifying
power. Thus a 2-inch objective is understood to be a combination which
has the magnifying power of a single lens whose focal point is two
inches from the object, and so on with reference to other powers.
By the aid of different eye-pieces an extensive range of magnifying
power can be obtained; for example, the 2-inch objective with a deep
eye-piece will give the same amplification as the quarter objective
with the ordinary eye-piece. Indeed, for certain observations, the
combination of a wide-angled low-power objective, with a deep
eye-piece, or _compensating eye-piece_, is considered to have an
advantage.
It has been already explained that two objectives, one of much greater
power than the other, but both having only the same numerical aperture,
will show only the same amount of _detail_; the higher power on a
larger scale. That is, supposing with a 1/4-inch objective of 1·0
numerical aperture certain structure is resolved, then a 1/8-inch
substituted with exactly the same numerical aperture, but with double
the magnification, no more _resolving power_ will be found in the
latter objective than in the former. For this reason a doubt has been
expressed as to whether high-power objectives--especially the more
expensive oil-immersions, made to transmit large pencils of light
through their larger apertures--are so well adapted for ordinary
research as the best series of dry achromatic objectives, or even,
in some instances, the medium aperture lenses; undoubtedly, for
histological (physiological and pathological) work, the latter will be
found to meet the students’ requirements quite as well as the former.
The student or amateur will do well to commence with moderate or medium
powers, a 2-inch, a 1-inch, a 1/2-inch, a 4/10-inch, or 1/4-inch.
These, together with the A and B eye-pieces, will give a range of
magnification from 30 to 250 diameters.
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