The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
down with a needle upon the liquefied balsam, the pressure being just
applied on one side rather than over the whole surface, so as to drive
the superfluous balsam towards the opposite side; finally, an equable
pressure over the whole will secure a perfect attachment of the section
without air bubbles. If, however, these should present themselves in
drying, and they cannot otherwise be expelled by pressure, it will
be found better to take the section off and relay it as before. The
thickness of the layer of balsam may be reduced by rubbing it down
before applying the glass-cover.
_Rock Sections._--Small pieces of rock may be ground down by the aid
of the lathe, or on a zinc plate, with emery powder and water, until
one side is rendered smooth and flat. Then fasten the polished side
of the section to a square of glass on the metal holder of the lathe,
with dried Canada balsam, as directed for bone, and allow it time to
become consolidated. When moderately thin take a piece of plate-glass
and some fine emery or putty-powder and rub the section down as thin as
possible. When found to be thin enough wash it well in water, and put
it aside to dry, or warm it over a spirit-lamp, and with a needle push
the section off the glass into a watch-glass of benzole or turpentine,
and allow it to soak until all the balsam is dissolved out. Wash
again in turpentine, and mount in Canada balsam, with or without a
cover-glass. Sections of echinus spines, shells, stones of fruits, &c.,
are prepared in the same way as those of bones and teeth; but when the
grinding is finished, the sections must be passed through alcohol into
oil of cloves, after which they should be mounted in Canada balsam. If
tolerably thin, sections of these substances can be cut in the lathe;
in the first instance, there will be no actual occasion to attach them
to glass at all, except for the purpose of obtaining a hold upon the
specimen for polishing, but the surface thus attached must afterwards
be completely removed in order to bring into view a stratum which the
Canada balsam may not have penetrated.
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