The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
With regard to smaller bodies, these can scarcely be treated in any
other way than by attaching a number of them to slips of glass at once,
and in such a way as to make them mutually support each other. Thus in
making horizontal and vertical sections of _foraminifera_, it would be
impossible to slice them through unless they were laid close together
in a bed of hardened Canada balsam, and first grinding away one side
and then turning and rubbing down the other. My friend, Dr. Wallich,
many years ago communicated to me the ingenious plan adopted by himself
when mounting and turning a number of these minute objects together.
The specimens being cemented with Canada balsam, in the first instance,
to a thin film of mica, and then attached to a glass slide by the same
means, when ground down to the thinness desired, the slide must be
gradually heated just sufficiently to allow of the detachment of the
mica-film and the specimen it carries; a clean slide with a thin layer
of hardened balsam having been prepared, the mica-film is transferred
to it with the ground surface downward. Its adhesion by drying having
been complete, the grinding and polishing should be proceeded with;
and as the mica-film will yield to the stone without any difficulty,
the specimen now reversed in position may be further reduced to the
requisite thickness for mounting as a permanent object.
_Staining and Mounting Vegetable Tissues._--Bacteria I propose to
treat of in a separate section. Vegetable tissues generally will first
receive attention, and their differentiation is based on the employment
of delicate gradations of colour stains. The more striking results are
obtained by _Multiple Staining_, while the cell contents are rendered
more palpable. On this account colouring media have been divided into
_nuclear_, _plasmic_, and _specific_. The first are chiefly valued in
proportion as they prove to have a selective affinity for the nuclei of
cells, and leaving the protoplasm comparatively unstained. Such stains
are needful for fresh and young tissues. On the other hand, _plasmic_
stains colour tissue uniformly, and are used to give a ground colour
by way of contrast; and _specific_ stains are chiefly employed to
distinguish certain elementary structures from the mass of cellulose
which forms the basis of vegetable tissue, and which is also met with
to a slight extent in animal membranes.
Public-domain text, read in full here on John Shaqi.
Reviews
Reviews
No reviews yet
Be the first to share your thoughts on this work.
Elsewhere in the archive
Join the Discussion
Join the discussion
Sign in to leave a comment or review.
Sign InorCreate an account