The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
_Cells for Mounting._--The minuter forms of life should be mounted in
thin cells, which may be readily made with Japanners’ gold size, dammar
or asphalte, and a Shadbolt or Walmsley’s turntable. The glass slide
being placed under the metal springs in such a manner that its two
ends shall be equi-distant from the centre (a guide to the position is
afforded by the circles traced out on the brass), take a camel’s hair
pencil and dip it into the Japanner’s gold-size, holding it firmly
between the finger and thumb, and set the wheel in motion, when a
perfect circle will be formed; put it aside to dry, or place it in the
warm chamber to harden. To cut cover-glasses place a sheet of thin
glass under the brass springs, and substitute for the pencil a cutting
diamond. A cutting diamond is not only useful to the microscopist for
the above purpose, but also for writing the names of mounted objects on
one end of the slide.
It will be found convenient to make a number of such cells, and keep
a stock ready for use. There are many objects whose structure is very
transparent. These should be mounted dry. Scales from the wings of
butterflies and moths, of the podura and lepisma, and some of the
diatomaceæ are of this class. All that is necessary in preparing
objects for dry mounting is to take care that they are free from
extraneous matter, and fix them permanently in the position in which
their structure will show to the best advantage.
For mounting specimens of greater thickness it is desirable to use
deeper cells. It will then be found convenient to make a second or a
third application of the gold-size, allowing sufficient time between
applications for the varnish to dry. Cells of a still deeper kind
are made up by cementing rings of glass or metal to the glass-slides
with marine glue or Brunswick black. The latter will be rendered more
durable by mixing in a small quantity of indiarubber varnish (made
by dissolving small strips of caoutchouc in gas-tar). The process
of mounting in glass-cells is similar to that employed in making
varnish-cells, except that a somewhat larger quantity of cementing
medium is required. Objects mounted in this way should be kept for a
time in the horizontal position, and a little fresh varnish must be
applied if the cement shows a tendency to crack. In mounting objects
in balsam, care must be taken to have the specimen _quite dry_ before
transferring it to turpentine. Objects mounted in cells should become
_perfectly saturated_ with the mounting fluid before being finally
cemented down.
[Illustration: Fig. 263.--Glass-cells for Mounting.]
It is preferable to mount and preserve specimens of animal tissues
in shallow cells, to avoid undue pressure on the preparation. Cells
intended to contain preparations immersed in fluid must be made of
a substance impervious to the fluid used, such as here represented
(Fig. 263). The surface of the fixed glass-circle should be slightly
roughened before applying the cement.
Public-domain text, read in full here on John Shaqi.
Reviews
Reviews
No reviews yet
Be the first to share your thoughts on this work.
Elsewhere in the archive
Join the Discussion
Join the discussion
Sign in to leave a comment or review.
Sign InorCreate an account