The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
Different modes of mounting may be employed with advantage; for
instance, entomological specimens, as legs, wings, spiracles, tracheæ,
ovipositors, stings, tongues, palates, corneæ, should be mounted in
balsam; the trachea of the house-cricket, however, should be mounted
dry. Sections of bone may either be mounted dry or in a fluid. Other
objects, as sections of wood and stones of fruit, exhibit their
structure best in Canada balsam.
In mounting entomological specimens, the first thing, of course,
is the dissection of the insect. This is best accomplished by the
aid of a dissecting microscope, a pair of small brass forceps, and
finely-pointed scissors; the parts to be prepared and mounted should
first be carefully detached from the insect with the scissors, then
immersed in a solution of caustic alkali (_liquor potassæ_) for a few
days, to soften and dissolve out the fat and soft parts. The length
of time necessary for their immersion can only be determined by
experience, but, as a general rule, the objects assume a certain amount
of transparency when they have been long enough in the alkali; when
this is ascertained, the object must be placed in a proper receptacle
and put by to soak for two or three hours in soft or distilled water.
It should then be placed between two slips of glass, and gently pressed
till the softer parts are removed. Should any adhere to the edge of
the object, it will be necessary to wash the specimen carefully in
water, a process that will be much assisted by the delicate touches
of a camel’s-hair brush. Place the object now and then under the
microscope to see that all extraneous matter is removed, and when this
is accomplished take the specimen up carefully with the camel’s-hair
brush, or a lifter, and place it on a piece of very smooth paper (thick
ivory note is the best for the purpose), arrange it carefully with the
brush and a finely pointed needle, place a second piece of paper over
it, and press it flat between two slips of glass, and compress it by a
small spring clip (Fig. 264). A dozen clips may be had for a few pence.
When _thoroughly_ dry (which it will probably be in about twenty-four
hours, if in a warm room), separate the glasses, and gently unfold the
paper; then, with a little careful manipulation, the object may be
readily detached, and placed in a little spirit of turpentine, where
it should be allowed to remain until rendered transparent and fit for
mounting. The time during which it should remain in this liquid will
depend on the structure; some objects, such as wings of flies, will be
quickly permeated, while horny and dense objects require an immersion
of a fortnight or even longer. A pomatum pot with a _concave_ bottom
and well-fitting lid will answer admirably for conducting the soaking
process in; and it is well, in preparing several specimens at a time,
to have two pots, one for large and medium, the other for very small
objects, otherwise the smaller will adhere to the larger.
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