The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
For mounting minute objects, carbolic acid solution will be found a
useful medium--the purest crystals of carbolic acid dissolved in just
sufficient water to render them fluid. No more should be dissolved than
may be wanted for the time being, as if left standing exposed to the
light it changes colour. Small crustacean foraminifera, the palates of
moluscs, after boiling a short time in liquid potash and well washing
to remove all traces of alkali, may be preserved in carbolic acid
solution. Should the specimens appear cloudy gently warm the slide over
a spirit lamp.
_Preserving and Killing Rotatoria with cilia in situ._--Mr. C.
Rousselet’s method of preserving and mounting the Rotatoria[47] has
been attended with so much success that the old difficulty attendant
upon the preservation of these various beautiful forms of infusorial
life has been practically overcome. The process resorted to consists
of four stages, namely, narcotising, killing, fixing, and preserving.
In dealing with rotifers hitherto, the difficulty has been that of
successfully killing them with their rotating organs fully extended.
It has been found needful to have recourse in the first instance to
a narcotising agent, and one that acts slowly. The most suitable is
a weak solution of the hydrochlorate of cocaine, a one per cent.
solution, or even weaker. This was first proposed by Mr. Weber for
keeping these active little bodies quiet while under observation. Mr.
Rousselet carries this agent further; he applied it to narcotise them
prior to killing, and this it does most effectually. The rotifers are
seen to sink to the bottom of the live-cell, and the cilia gradually
to slacken in motion, and the time for killing has arrived. This is
effected by Flemming’s chromo-aceto-osmic acid. A rather weak solution
must be employed--consisting of 1 per cent. solution of chromic acid,
15 parts; 2 per cent. osmic acid, 4 parts; glacial acetic acid, 1
part--which is at the same time a killing and fixing medium. The word
“fixing” must not be taken to imply simply fixing, as it includes
rapidly _killing_ and _hardening_ and preventing further change in
the tissues of the rotifers by subsequent treatment, as mounting. The
animal, therefore, must remain quietly for a few minutes, and then
taken out and washed in five or six changes of distilled water, and
hence transferred to the preservative fluid. All this must be effected
with great care. The best preservative fluid is simply distilled water,
rendered antiseptic by a trace of the fixing solution (about eight
drops to an ounce of water) giving the slightest tinge of yellow to
the solution. This slight tinge of colour is imparted to the rotifers,
otherwise they remain transparent and unchanged, while the nervous
tissue throughout the body is brought out to perfection.
Public-domain text, read in full here on John Shaqi.
Reviews
Reviews
No reviews yet
Be the first to share your thoughts on this work.
Elsewhere in the archive
Join the Discussion
Join the discussion
Sign in to leave a comment or review.
Sign InorCreate an account