The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
A useful cement for fixing minute objects, diatoms, &c., temporarily to
thin glass covers, before permanently mounting them in Canada balsam,
is made as follows:--Dissolve, without heat, two or three grains of gum
arabic in one ounce of distilled water, then add glacial acetic acid,
three minims, and the least trace of sugar. Filter carefully through
filter paper, and repeat this in the course of three or four weeks.
This cement will be unaffected by the balsam.
_Mounting Chara._--It is often found difficult to preserve and mount
the fruit of chara, but this can be successfully accomplished in
glycerine jelly, by taking the following precautions. After cleaning
the specimen place it in 92 per cent. of alcohol for several hours,
then transfer it to a mixture of equal parts of spirit and glycerine
for several hours longer, pour off nearly all the mixture, and add pure
glycerine at intervals till the glycerine becomes concentrated. The
specimen is then mounted in glycerine jelly in a cell just deep enough
to take it without pressure.
There are some objects much more difficult to prepare than others, and
which tax the patience of the beginner in a manner which can hardly
be imagined by any one who has never made the attempt. The structure
of many creatures is so delicate as to require the very greatest care
to prevent mutilation, and consequent spoliation, of the specimen.
The beginner, therefore, must not be discouraged by a few failures in
commencing, but should persevere in his attempts, and constant practice
will soon teach him the best way of managing intricate and difficult
objects. The room in which he operates should be free from dust, smoke,
and intrusion, and everything used should be kept scrupulously clean,
since a very small speck of dirt, which may be almost invisible to the
naked eye, will assume unpleasant proportions under the microscope, and
not only mar, but possibly spoil a fine and delicate preparation.
Few students on commencing to work with the microscope will fully
realise the fact that under medium or high powers the natural
appearance of almost all objects is changed by the refractive nature of
the fluid medium in which they are immersed and which enters more or
less into their composition. The remarkable changes effected by the law
of diffusion, when alkaloid substances enter into their composition,
show the necessity of taking every precaution in the employment of
preservative fluids. Glycerine affords an example of the chemical
change induced, should the preparation have been passed through an
alkaline solution.
_Air Bubbles_ are a constant source of annoyance both in preparing and
mounting. These may be removed from the specimen by gently warming the
under part of the slide over a spirit lamp, or placing the slide in
the warm chamber, when the bubbles will move towards the edge of the
cover-glass and ultimately disappear. The air-pump is preferred by many
microscopists.
Collection of Objects.
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