The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
In the early part of this century the optical correction of chromatic
aberration was partially brought about by combining a convex lens of
crown-glass with a concave lens of flint-glass, in the proportion of
which these two kinds of glass respectively refract and disperse rays
of light; so that the one medium may by equal and contrary dispersion
neutralise the dispersion caused by the other, without at the same
time wholly neutralising its refraction. It is a curious fact that the
media found most available for the purpose should be a combination of
crown and flint-glass, of _crown-glass_ whose index of refraction is
1·519, and dispersive power 0·036, and of _flint-glass_ whose index
of refraction is 1·589, and dispersive power 0·0393. The focal length
of the convex crown-glass lens must be 4-1/3 inches, and that of the
concave flint-glass lens 7-2/3 inches, and the combined focal length 10
inches. The diagram (Fig. 19) shows how rays of light are brought to a
focus, nearly free from colour. The small amount of residual colour in
such a combination is termed the _secondary spectrum_; the violet ray
F Y, crossing the axis of the lens at V, and going to the upper end P
of the spectrum, the red ray F B going to the lower end T. But as the
flint-glass lens _l l_, on the prism A _a_ C, which receives the rays
F V, F R, at the same points, is interposed, these rays will unite at
_f_, and form a small circle of white light, the ray S F being now
refracted without colour from its primitive direction S F Y into the
direction F _f_. In like manner, the corresponding ray S F′ will be
refracted to _f_, and a white colourless image be the result.
[Illustration: Fig. 19.--Correction of Chromatic Aberration.]
The achromatic aplanatic objective constructed on the optical formula
enunciated, did not meet all the difficulties experienced by the
skilled microscopist, in obtaining resolution of the finest test
objects, and whereby the intrinsic value of the objective (in his
estimation) must stand or fall. There were other disturbing residuary
elements besides those of the secondary spectrum, and which at a later
period were met by the practical skill of the optician, who applied the
screw-collar, and by means of which the back lens of the objective is
made to approach the front lens, thus more accurately shortening the
distance between the eye-piece, where the image is eventually formed,
and the back lens of the objective.
In this diagram L L is a _convex_ lens of _crown-glass_, and _l l_ a
_concave_ one of _flint-glass_. A convex lens will refract a ray of
light (S) falling at F on it exactly in the same manner as the prism A
B C, whose faces touch the two surfaces of the lens at the points where
the ray enters, and quits. The ray S F, thus refracted by the lens L
L, or prism A B C, would have formed a spectrum (P T) on a screen or
wall, had there been no other lens.
[Illustration: Fig. 20.--Virtual Image formed by Convex Lens.]
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