It has been said that no comparison can be instituted between microscopic vision and macroscopic; that the images formed by minute objects are not delineated microscopically under ordinary laws of diffraction, and that the results are dioptrical. This assertion, however, cannot be accepted unconditionally, as will be seen on more careful examination of the late Professor Abbe’s masterly exposition of “The Microscopical Theory of Vision,” and also his subsequent investigations on the estimation of aperture and the value of wide-angled immersion objectives, published in the “Journal of the Royal Microscopical Society.” The essential point in Abbe’s theory of microscopical vision is that the images of minute objects in the microscope are not formed exclusively on the ordinary _dioptric_ method (that is, in the same way in which they are formed in the camera or telescope), but that they are largely affected by the peculiar manner in which the minute construction of the object breaks up the incident rays, giving rise to _diffraction_. The phenomena of diffraction in general may be observed experimentally by plates of glass ruled with fine lines. Fig. 26 shows the appearance presented by a single candle-flame seen through such a plate, an uncoloured image of the flame occupying the centre, flanked on either side by a row of coloured spectra of the flame, which become dimmer as they recede from the centre. A similar phenomenon may be produced by dust scattered over a glass plate, and by other objects whose structure contains very minute particles, or the meshes of very fine gauze wire, the rays suffering a characteristic change in passing through such objects; that change consisting in the breaking up of a parallel beam of light into a group of rays, diverging with wide angle and forming a regular series of maxima and minima of intensity of light, due to difference of phase of vibration.[10] [Illustration: Fig. 26.] In the same way, in the microscope, the diffraction pencil originating from a beam incident upon, for instance, a diatom, appears as a fan of isolated rays, decreasing in intensity as they are further removed from the direction of the incident beam transmitted through the structure, the interference of the primary waves giving a number of successive maxima of light with dark interspaces.