The confusion into which the aperture question at this period had lapsed was no doubt due to the fact that its opponents had not yet grasped the true meaning of the term _aperture_. It was believed to be synonymous with “angular aperture,” much in use at the time. It will, however, appear quite unaccountable that even the older opticians should have confounded the latter with the former; and so entirely disregarded the fact that the angles of the pencil of light admitted by the objective cannot serve as a measure of its _aperture_, and that high refractive media can greatly reduce the value length of waves of light. When the medium in which the objective works is the same as air, it is not that a comparison can be made by the angles of the radiant pencils only, but by their sines. For example, if two dry objectives admit pencils of 60° and 180°, their real apertures are not as 1 : 3, but as 1 : 2 only. Aperture in fact is computed by mathematicians by tracing the rays from the back focus through the system of lenses to the front focus, the front focus being the point at which the whole cone of rays converge as free as may be from aberration. If the front focus be in air, no pencil greater than 82°, “double the angle of total reflection,” can _emerge_ from the plane front of the lens; and, obviously, if no greater cone can emerge to a focus one way, neither can any greater cone enter the body of the lens from the radiant. This angle, then, of 82°, must be regarded as the limit for dry lenses or objectives. This limit, it will be seen on more careful examination, is very nearly the maximum angle that can be computed for a lens to have a front focus in air. This can be proved by the consideration of the angle of the image of rays, as they are radiated from the object itself in balsam: for although this angle of image rays viewed as nascent from a self-luminous object capable of scattering rays in all directions, may be 180° in the substance of the balsam and cover-glass, of the 180° only 82° of the central portion will emerge into air--all rays beyond this limit are internally reflected at the cover-glass. This cone, then, of 82° becomes 180° in air, and a large part must necessarily be lost by reflection at the first incidence on the plane front of the lens. But with a formula permitting the use of a water medium between the front lens and the cover-glass, the aperture of the image rays may reach 126°--double the critical angle from glass to water; and with an oil medium, the aperture will be found to be limited only by the form of the front lens that can be constructed by the optician. To sum up, then, the effect of the immersion system, greatly assists in the correction of aberration, gives increased magnification and angular aperture, increase of working distance between the objective and object, and renders admissible the use of the thicker glass-cover.