It is not necessary to give further details bearing on the three additional experiments. It will suffice to quote the general conclusions which Munk drew from the various results obtained, viz., that a low proteid intake in the case of dogs causes a loss of appetite, weakness, vomiting, etc., while body-weight and nitrogen equilibrium are difficult or impossible to maintain. More specifically, Munk’s observations led him to state that for dogs of ten kilograms body-weight a daily intake of 0.255 gram of nitrogen per kilo of body-weight is not sufficient to maintain the normal condition of the body, even when the fuel value of the day’s food amounts to more than 100 calories per kilo. In order to have the animal continue in nitrogen and body equilibrium, the daily food must contain at least 0.31 gram of nitrogen per kilogram of body-weight, with sufficient non-nitrogenous food to yield over 100 calories per kilo. Let us now pass to the experiments made by Rosenheim,[69] which were carried on at about the same date as Munk’s. In the first experiment, the dog weighed 11.3 kilograms, and was fed daily a low proteid ration having a fuel value of 1447 calories and containing 2.825 grams of nitrogen. This ration was reduced in a short time to a still lower plane, viz., to 1066 calories and 2.525 grams of nitrogen daily. The food as then given was composed of 170 grams of rice, 50 grams of fat, and 25 grams of chopped meat, on which the dog gained weight and preserved nitrogen equilibrium. For six weeks, or thereabouts, the animal maintained its normal condition, after which it began to show symptoms of a general disturbance, with lack of appetite and weakness accompanied by a condition of icterus. Addition of meat extract to the diet to improve the flavor was without any appreciable effect. During the next two weeks, the condition of the animal steadily grew worse, although the body-weight remained practically stationary and nitrogen equilibrium was maintained. A week later, the animal died in a condition of exhaustion, without having manifested any symptoms of disturbed metabolism. There was found a marked catarrhal condition of the mucous membrane of the gastro-intestinal tract, with a fatty degeneration or metamorphosis of the glandular apparatus, but nothing sufficiently specific to account for the peculiar manner of death. [69] Theodor Rosenheim: Ueber den Gesundheitsschädigenden Einfluss eiweissarmer Nahrung. DuBois-Reymond’s Archiv für Physiologie, 1891, p. 341. Also, Weiterer Untersuchungen über die Schädlichkeit eiweissarmer Nahrung. Pflüger’s Archiv f. d. gesammte Physiologie, Band 54, p. 61, 1893.