Upon these observations the Calcutta municipality felt encouraged to vote funds for the continuance of the inoculations in an experimental farm, and appointed for that purpose a special staff. In 1896 the result of two years’ observations were embodied by the health officer in a report to the Calcutta Municipal Corporation. It recorded a most satisfactory state of affairs. During the time under observation some eight thousand persons were inoculated. Cases of cholera occurred in seventy-seven huts in which some members of the family had been previously inoculated and others not. Comparing the incidence of the disease in the two groups, a striking advantage was found to be with the inoculated. I made an analysis of the cases according to the time which had elapsed between inoculation in each of these huts and the occurrence of cholera in them, and the following results were found. During the first four days after inoculation, apparently before the vaccine had time to produce its full protective effect, there were proportionately 1.86 times fewer deaths among the inoculated than among the non-inoculated members of the families. In a second period, extending from the fifth to the four hundred and twenty-ninth day--i. e., for fourteen months--there were 22.62 times fewer deaths among the inoculated; while in the last period--that is, between the four hundred and thirtieth and seven hundred and twenty-eighth day after the inoculation--there were only 1.54 fewer deaths among the inoculated, the immunity having evidently gradually disappeared. The net result was that for two years after inoculation, including the periods of incomplete protection, there was a reduction in mortality of 72.47 per cent among the inoculated; or in other words, in houses in which inoculations were performed and in which cholera subsequently occurred there were, even from the day of inoculation, before the full effect of it could be produced, eleven deaths among the non-inoculated to only three among the inoculated. Eight lives out of every eleven were saved.