19. Of course, during the whole process the injecting fluid and subject must be kept at a temperature high enough to allow the liquid to flow freely; and the escape of a little of it need cause no fears to the student, as it is almost impossible to fill any subject without some loss. When the injected object has received sufficient fluid, it should have a plump appearance, owing to all the vessels being well filled. The vessel must then be tied up where the pipe was inserted, and the whole left in cold water two or three hours, after which time it may be mounted; but it may be well to notice a few things which the beginner ought to know before entering into that part of the process; and he may be here informed that it is not necessary to mount the objects immediately, otherwise it would be impossible for one person to make use of half of any large subject, as it would be in a state of decay long before each part could have been examined and separated. Large pieces should be therefore immersed in equal parts of spirits of wine and water, or glycerine, which some think better still, and thus preserved in bottles until time can be given to a closer examination. 20. In operating upon large subjects, entire animals, &c., the constant pressure required by the piston of the syringe grows wearisome, besides occupying both hands, which is sometimes inconvenient when working without assistance. To obviate this, another way of driving the syringe was published in the “Micrographic Dictionary” which I will quote here:--“We have therefore contrived a very simple piece of apparatus, which any one can prepare for himself, and which effects the object by mechanical means. It consists of a rectangular piece of board, two feet long and ten inches wide, to one end of which is fastened an inclined piece of wood (equal in width to the long board, and one foot high). The inclined portion is pierced with three holes, one above the other, into either of which the syringe may be placed--the uppermost being used for the larger, the lowermost for the smaller syringe; and these holes are of such size as freely to admit the syringe covered with flannel, but not to allow the rings to pass through them. The lower part of the syringe is supported upon a semiannular piece of wood, fastened to the upper end of an upright rod, which slides in a hollow cylinder fixed at its base to a small rectangular piece of wood; and by means of a horizontal wooden screw, the rod may be made to support the syringe at any height required. The handle of the syringe is let into a groove in a stout wooden rod connected by means of two catgut strings with a smaller rod, to the middle of which is fastened a string playing over a pulley, and at the end of which is a hook for supporting weights, the catgut strings passing through a longitudinal slit in the inclined piece of wood.” When in use the syringe is filled with injecting fluid, and passed through one of the three holes which