The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
is most suitable. The object being placed so that the pipe and syringe
can be best joined, the rod and strings are set in order, and a weight
placed on the hook. The stopcock must then be opened gradually, when
the operator will be able to judge whether the weight is a proper one
or not: if the piston is driven with any speed, there is danger of
injuring the subject, and less weight may be used; if, however, the
piston does not move, more must be added.
21. Such is the method recommended by the “Micrographic Dictionary,”
and perhaps it is as good as any mechanical plan could be; but where
the operator is willing to undergo the labour of performing all this
with the hand, he has a much better chance of succeeding, because the
pressure can be regulated so accurately, and changed so quickly when
requisite, that no mere machine can compete with it, however well
contrived.
22. When the beginner attempts to inject a subject, one of his
difficulties is finding the vessel from which to commence. Another
consists in distinguishing the arteries from the veins; but this is
partly removed by making a longitudinal incision in the vessel, and
with a blunt thick needle probing a little distance into the tube. The
artery will be found thicker in the coating than the vein, and the
difference is easily perceived by this mode of testing: the vein is
also of a bluer colour than the artery. I say above, a “longitudinal
incision” must be made: the reason for this is, the artery when cut
across contracts considerably, and is lost in the adjoining substance;
but where the opening is made _longitudinally_ all danger of this
contraction is obviated.
23. The different systems of vessels are often injected with various
colours, so that their relative positions, &c., may be shown most
clearly. In some specimens, the veins are injected with white, and the
arteries with red; in the kidney, the urinary tubes are often filled
with white, and the arteries with red. Then, again, the liver affords
tubes for three or four colours. But no written instructions on this
point can benefit the young student, and he must be content for a
while to employ himself with single colours until he has gained the
mechanical skill and the primary knowledge which are necessary before
he can make any advance.
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