[G] When, however, it is desirable to cut very thin sections of the injected subject, the carmine is sometimes added to a solution of fine gelatine--gelatine one part to water eight parts. But the warm water and mode of proceeding which are used with the size solutions before described will be necessary in this case also. 33. The method of making injections with these colours is the same as with the gelatine mixtures before described, except that no heat is required, and consequently most of the trouble removed. The bottle of the fluid must be well shaken immediately before use; and when the object is injected, we must allow it to remain in a cool place for a few hours before cutting it. Thin sections of the subject may be cut with Valentine’s knife, as before described, and are very beautiful transparent objects. Some of the finer tissues, also, are shown much better by this mode of injection than by the opaque, and are easily mounted by washing in clean water when first separated, and floating upon a slide, where they must be allowed to dry thoroughly. They may then be immediately mounted in balsam, or kept in the dry state until it is convenient to finish them; but in many cases this keeping, if too much prolonged, will injure the object. If it is desired to transfer the section to another slide, it will be necessary to wet it thoroughly with water by the aid of a camel-hair pencil, and then gently strip it off with the forceps. When it is wished to preserve injected subjects in “masses,” it must be done by immersion in spirit, and the sections may be cut at leisure. Most of these transparent objects may be mounted in Canada balsam; but some recommend glycerine or glycerine jelly, as allowing the use of a higher power in their examination, and preserving them in a more natural form.