The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
Glycerine 1 ounce.
Wood naphtha, or pyroacetic acid 1½ drachm.
Spirits of wine 1 ounce.
Ferrocyanide (yellow prussiate) of potash 12 grains.
Tincture of sesquichloride of iron 1 drachm.
Water 4 ounces.
Dissolve the ferrocyanide of potash in one ounce of the water;
add the tincture of sesquichloride of iron to another ounce. Mix
these solutions gradually together, shaking the bottle well which
contains them--it is best to add the iron to the potash solution.
When thoroughly mixed, these solutions should produce a dark-blue
mixture, perfectly free from any perceptible masses or flocculi. Next
mix the naphtha and spirits of wine, and add the glycerine and the
remaining two ounces of water. This must now be slowly mixed with the
blue liquid, shaking the whole well in a large bottle whilst the two
come together. The tincture of sesquichloride of iron is recommended,
because it can always be obtained of a uniform strength.
31. Dr. Turnbull used a mixture slightly different from the above,
which is made with the sulphate of iron:--
Purified sulphate of iron 10 grains.
Ferrocyanide of potassium 32 grains.
Glycerine 1 ounce.
Pyroacetic acid 1½ drachm.
Alcohol 1 ounce.
Water 4 ounces.
Dissolve the sulphate of iron in one ounce of the water, gradually add
the ferrocyanide of potassium dissolved in another ounce, and proceed
as above.
32. Dr. Beale also gives us the following carmine injection to be
employed in the same way as the blue.[G] Take--
Carmine 5 grains.
Glycerine, with 8 or 10 drops of hydrochloric acid ½ ounce.
Glycerine (pure) 1 ounce.
Alcohol 2 drachms.
Water 6 drachms.
Mix the carmine with a few drops of water, and when well incorporated
add about five drops of liquor ammoniæ. To this dark-red solution about
half an ounce of the glycerine is to be added, and the whole well
shaken in a bottle. Next, very gradually pour in the acid glycerine,
frequently shaking the bottle during admixture. Test the mixture with
blue litmus-paper, and if not of a very decidedly acid reaction, a few
more drops of acid may be added to the remainder of the glycerine and
mixed as before. Lastly, mix the alcohol and water very gradually,
shaking the bottle thoroughly after adding each successive portion
till the whole is mixed. This fluid may be kept ready prepared, and
injections made very rapidly with it.
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