The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
Tadpoles of the frog (which, of course, are only obtainable in their
season) are good subjects for showing the circulation of the blood.
They are best suited for the microscope when about one inch long. The
tadpoles of the newt and toad also are equally suitable. They may
be placed in a very shallow glass trough with a little water, and a
narrow band of linen bound lightly round in some part not required for
examination, to keep them from moving; or they may be laid upon a glass
plate with a drop or two of water, and a thin glass covering lightly
bound upon it. Dr. Carpenter, however, places them first in cold water,
gradually adding warm until the whole becomes about 100°, when the
tadpole becomes rigid, whilst the circulation is still maintained. I
have not, however, found this necessary, the thin glass accomplishing
all that is desired. The tail is generally the most transparent, and
shows the circulation best; but in some of the newt larvæ the blood may
be traced down to the very extremities if they are not _too old_. Mr.
Whitney places the tadpole upon its back, by which means the heart and
other internal arrangements may be seen.
Amongst fishes also may be found subjects for the same purpose, but
they seldom furnish as good examples as those before mentioned, because
the blood-vessels are not nearly so abundant, as in the foot of the
frog, &c. The stickleback is, however, procurable almost in any place
during the summer months, and may be laid in a shallow trough, loosely
bound down as the tadpole. The tail may be covered with a piece of thin
glass to prevent him curling it to the object-glass. The power needed
with this will be about the same as with the other subjects--viz., half
to quarter inch object-glass.
It is not absolutely necessary to go to reptiles or fishes for this
curious sight, as some other animals serve very well. In the wings of
the common bat may be found a good subject. These must be stretched out
on something resembling the frog-plate before described, when those
parts near to the bones will show the _largest_ vessels very clearly.
The ear of a young mouse is an illustration of the same phenomenon, but
it is very difficult to fix it in a good position, as these animals are
so very timid and restless.
Amongst insects also the same law may be observed, by placing them in
the “cage,” or “live-box,” so as to keep them still, but not to injure
them by too much pressure. In certain larvæ this is particularly well
shown, as that of the day-fly and plumed gnat; but in some of these
the blood is almost colourless. In the wings also of many insects this
circulation is well seen, as in those of the common housefly; but as
these parts become dry in a few days, the subject should not be more
than twenty-four hours old.
Public-domain text, read in full here on John Shaqi.
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