The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
Somewhat approximating to the forementioned phenomenon, is the
“rotation” of fluid in the cells, or, as it is usually termed, the
_circulation of the sap_, of plants. This is shown in certain vegetable
growths as a constant stream of thick fluid, wherein small globules
are seen; which stream flows round the individual cells, or up the
leaf, turning at the extremity, and down again by a different but
parallel channel. There is little or no difficulty in showing this in
many plants; but some are, of course, better than others, and require
a different treatment; we will, therefore, notice a few of these.
Perhaps the best of all is the _Vallisneria spiralis_, which is an
aquatic plant, frequently grown in, but not really belonging to, this
country. As it somewhat resembles grass, the leaf is not used in its
natural state, but a thin section cut lengthwise with a razor or other
sharp instrument--this section, however, is much better when the outer
surface has been first removed. It should then be laid upon a slide
with a drop or two of water, and covered with a piece of thin glass.
Often the cutting of the section seems to be such a shock to the leaf
that no motion is visible for awhile, but in a short time the warmth
of an ordinary sitting-room will revive it, and with a quarter-inch
object-glass the currents will be rendered beautifully distinct.
Where the “stream” is unusually obstinate the warmth may be slightly
increased, but too great heat destroys the movement altogether. In the
summer, any of the leaves show this “circulation” very well; but in the
winter, the slightly yellow ones are said to be the best.
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