The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
When ordinary daylight is employed for this purpose, a dark slide
will be required for the prepared plate, in the same way as for
photographing landscape, &c. These dark slides are generally made by
each individual to suit his particular arrangements of negatives, &c.;
but it may be here recommended that the operator should always focus in
the same slide which he is about to use, as so small a difference in
distance lies betwixt perfection and failure.
For an ordinary student, perhaps the above method is that which is the
most readily used, and consequently the most generally available; but
almost every one has a different arrangement of microscope, &c., by
which he procures these minute pictures. Mr. Shadbolt (one of our most
successful photographers) gives the following instructions:--“Having
removed the upper stage plate of a large compound microscope, I replace
it with one of wood, supplied with guide pins of silver wire, in order
to admit of its supporting a slip of glass coated with collodion, and
excited in the nitrate of silver bath in the usual way. If the ordinary
brass stage plate were left undisturbed, it is obvious that it and the
excited slip of glass would be mutually destructive.
“The microscope is now to be placed in a horizontal position, the
objective, intended to produce the picture, made to occupy the place
usually filled by the achromatic condenser on the _sub-stage_ of the
microscope, while _another_ objective is screwed into the lower end of
the body of the instrument, which is used not only to focus with, but
also to make the requisite allowance for actinic variation.
“The negative intended to be reduced is then arranged vertically, with
its centre in the axis of the microscopic body, at a distance of from
two to four feet from the lower object-glass, and with a convenient
screen of card, wood, or thick paper, to cut off any extraneous light
that would otherwise pass beyond the limits of the picture.
“A small camphine-lamp is employed for the purpose of illuminating the
negative, having a good bull’s-eye lens as a condenser, so arranged
with its flat side next the lamp that the refracted rays shall just
fill the whole of a double convex lens of about six inches in diameter,
the latter being placed in such a position as to refract the rays of
light in a parallel direction upon the negative. By this arrangement
the _bull’s-eye lens_ of about two inches and a half in diameter
_appears_ as the source of the light instead of the small flame of the
lamp.
“By using a bat’s-wing gas-burner of a good size, a _single_ lens,
instead of the two, may be so placed as to give the necessary
uniformity of illumination.”
This arrangement requires the same care in working as that before
mentioned, the pictures being produced, developed, and fixed by the
same treatment.
Public-domain text, read in full here on John Shaqi.
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