As before stated, almost every manipulator makes some small changes in the method of producing these minute pictures; but the rules given, though far from new, are sufficient for all purposes; and I may state with truth, that those which I procured when these wonders were quite new, are fully equal in every respect to the best usually met with at the present time. With these instructions I shall close my Handbook, as I believe that every branch of Preparation and Mounting of Microscopic Objects has been treated of. Not that the beginner can expect that he has nothing to do except read this to be able to mount everything; but there are difficulties from which he may be freed by instruction, when otherwise he would have been compelled to learn by failure alone. I may, here, however, repeat certain advice before given,--that, when practicable, it is a good thing to mount each object by two or more different methods, as very frequently one feature is best shown dry, another in liquid, and a third in balsam. Secondly, let the _mounting_ be studied thoroughly, as no part of the microscopic science is more worthy of thought than this. And lastly, let no failures prevent you following up what will assuredly one day become a source of great pleasure, and render your daily “constitutional walk,” which is often dull in the extreme, very delightful, as it will afford you some new wonder in every hedge-row. INDEX. Air-bubbles, 56. Air-pump, 13; use of, 57. Alcock, Dr., on tongues of Mollusca, 118. Algæ, mounting of, 91. Anacharis alsinastrum, rotation in, 143; to cultivate, 144. Animal tissues, dissection of, 114. Antennæ of insects, 71; in preservative liquid, 94. Apparatus required in mounting objects, i. Arteries, how to distinguish, in injection, 131. Asparagus, spiral vessels of, 114. Asphaltum, 15; and india-rubber, as cement and varnish, 18. Bat, circulation of blood in wings of, 142. Beale’s, Dr., Prussian blue for injecting, 134; carmine, 135. Bell-glasses, use of, 12. Berg-mehl, 40. Bermuda earth, 40. Bichromate of lead, injection with, 132. Bird, Dr. Golding, on preparation of Zoophytes, 65. Black-japan, as a cement, 16. Black varnish, 18. Blood, as a microscopic object, 50; circulation of, 140–142. Bone, sections of, 102; fossil, 103. Brass plate for heating glasses, &c., 13. Cactaceæ, raphides of, 52. Camel-hair pencils, use of, 10. Canada balsam, 14; with chloroform, 14, 60, 132; with turpentine, 14; air bubbles in, 56; Dr. Carpenter’s syringe for, 59; to fill cells with, 60; mounting of objects in, 56. Carbolic acid, 86. Carmine injecting fluid, Dr. Beale’s, 135: Dale & Davies’, 138. Cartilage, dissection of, 114. Castor oil, as a preservative, 86.