The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
Amongst the various classes of microscopic objects now receiving
general attention, the Diatomaceæ may be placed in a prominent
position. They afford endless opportunities of research, and some
very elaborate works have already been issued concerning them.
Professor Smith’s may be mentioned as one containing, perhaps, the
best illustrations. The young student may wish to know what a diatom
is. The “Micrographic Dictionary” gives the following definition:--“A
family of confervoid Algæ, of very peculiar character, consisting of
microscopic brittle organisms.” They are now looked upon by almost all
of our scientific men as belonging to the _vegetable_ kingdom, though
some few still assign them to the animal. They are almost invariably
exceedingly small, so that the unaided eye can perceive nothing on
a prepared slide of these organisms but minute dust. Each separate
portion, which is usually seen when mounted, is termed a “frustule,”
or “testule:” this consists of two similar parts, composed of silica,
between and sometimes around which is a mass of viscid matter called
the “endochrome.” They are found in almost every description of water,
according to the variety: some prefer sea-water, others fresh, and
many are seen nowhere but in that which is a mixture of both, as the
mouths of rivers, &c. Ditches, ponds, cisterns, and indeed almost every
_reservoir_, yield abundance of these forms. They are not, however,
confined to “present” life; but, owing to the almost indestructible
nature of their siliceous covering, they are found in a fossil state
in certain earths in great abundance, and are often termed “fossil
Infusoria.” Upon these frustules are generally to be seen lines,
or “markings,” of different degrees of minuteness, the delicacy of
which often serves the purpose of testing the defining power of the
object-glasses. Some of the frustules are triangular, others circular,
and, indeed, of almost every conceivable shape, many of them presenting
us with exquisitely beautiful designs.
The markings, however, are seldom seen well, if at all, until the
frustules are properly prepared, the different methods of accomplishing
which will be given a little further on.
Public-domain text, read in full here on John Shaqi.
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