The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
Many diatoms are seen best when mounted in a dry state, the minute
markings becoming much more indistinct if immersed in liquid or balsam;
and for this reason those which are used as test objects are usually
mounted _dry_. Many kinds also are now prepared in this way, as opaque
objects to be examined with the lieberkuhn, and are exquisitely
beautiful. Others, however, are almost invariably mounted in balsam;
but as these will be again referred to in Chapter III., and require
the same treatment to fit them for the slide, it will not be out of
place to describe the cleaning and preparation of them here. As before
stated, there is much matter surrounding them which must be got rid of
before the “_siliceous_ covering” can be shown perfectly. As, however,
we may first wish to become acquainted in some degree with what we have
to do, it is well to take a small piece of _talc_, and place a few of
the diatoms upon it. This may be held over the flame of the spirit-lamp
until all the surrounding matter is burnt away, and a tolerable idea
may be thus obtained as to the quality of our treasure.
In some cases it is well to use this burning operation alone in
_mounting_ specimens of diatoms, when they may be placed in their
natural state upon the thin glass, burnt for awhile upon the platinum
plate, hereafter described, and mounted dry or in balsam.
In the preparation and cleaning of Diatomaceæ, there is little
satisfaction unless these operations have been successfully performed,
as a very small portion of foreign matter seriously interferes with
the object. The mode of preparing them varies even amongst the most
experienced. It will be found, therefore, most satisfactory to examine
the principal of these separately, although it may be at the risk of
some little repetition.
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