The preparation & mounting of microscopic objects — John Shaqi
The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
It was before stated that the diatoms when cleansed might be preserved
in small phials of distilled water. When required for mounting, shake
the phial, and with a thin glass tube or rod take up a drop of the
fluid and spread it upon the surface of the slide in the desired
position. This must then be allowed to dry gradually, or by the aid
of the lamp if necessary, without being shaken or interfered with,
otherwise uniformity of dispersion will be prevented. When _thoroughly_
dry, a thin ring of one of the adhesive varnishes--gold-size will be
found as good as any--may be drawn round the diatoms, and allowed to
dry in a slight degree. The slide and thin glass cover should then be
warmed and the latter gently pressed upon the ring of varnish until the
adhesion all round is complete.
As some of the diatoms require object-glasses of extremely high power,
and, consequently, short focus, to show them, they must be as close to
the outer surface of the cover as possible. For this reason they are
sometimes placed upon the under side of the thin glass, as follows.
Clean the surfaces of the slide and cover, and with the rod or pipe
place the liquid containing the diatoms upon the thin glass, and dry as
before. Trace the ring to receive the cover upon the slide, and when
almost dry, warm both and proceed as above. Whichever of these methods
is employed, the outer ring of coloured varnish may be applied as
elsewhere described and the slide finished.
The diatoms are also sometimes mounted betwixt two thin glasses, as
described in Chapter I., so that the light by which they are examined
may receive as little interference as possible, and that an achromatic
condenser may be brought into focus under the slide.
Of the various modes of cleaning and mounting the Diatomaceæ, I believe
that the following may be safely recommended as affording results of
the very best quality. My friend, Mr. T. G. Rylands, gave it to me as
that which he prefers, and I can safely say that his numerous slides
are at least equal to any I have ever seen. I will give it just as I
received it from him, though there may be some little repetition of
what has been said elsewhere, as he does not appropriate any part of it
as his own. He says:--In this branch of mounting, general rules alone
can be laid down, because the gatherings may contain iron, lime, fine
silt, or vegetable matter under conditions for special treatment, and
consequently the first step should be to experiment on various kinds.
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