The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
When fresh from the dealer’s hands, these slides are generally
covered with dust, &c., which may be removed by well washing in clean
rain-water; but if the impurity is obstinate, a little washing soda
may be added, care being taken, however, that every trace of this is
removed by subsequent waters, otherwise the crystals will afterwards
form upon the surface. A clean linen cloth should be used to dry the
slides, after which they may be laid by for use. Immediately, however,
before being used for the reception of objects by any of the following
processes, all dust must be removed by rubbing the surface with clean
wash-leather or a piece of cambric, and, _if needful_, breathing upon
it, and then using the leather or cambric until perfectly dry. Any
small particles left upon the surface may generally be removed by
blowing gently upon it, taking care to allow no damp to remain.
We have before said, that any object to be viewed in the microscope
must have its support; but if this object is to be preserved, care
must be taken that it is defended from the dust and other impurities.
For this purpose it is necessary to use some transparent cover, the
most usual at one time being a plate of mica, on account of its
thinness; this substance is now, however, never used, thin glass being
substituted, which answers admirably. Sometimes it is required to
“_take up_” as little space as possible, owing to the shortness of
focus of the object-glasses. It can be procured of any thickness, from
one-fiftieth to one-two-hundred-and-fiftieth of an inch. On account
of its want of strength it is difficult to cut, as it is very liable
to “_fly_” from the point of the diamond. To overcome this tendency
as much as possible, it must be laid upon a thicker piece, previously
made wet with water, which causes the thin glass to adhere more firmly,
and consequently to bear the pressure required in cutting the covers.
The process of cutting being so difficult, especially with the thinner
kinds, little or nothing is gained by cutting those which can be got
from the dealers, as the loss and breakage is necessarily greater in
the hands of an amateur. It is convenient, however, to have on hand a
few larger pieces, from which unusual sizes may be cut when required.
Public-domain text, read in full here on John Shaqi.
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