The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
In mounting these objects, some are so delicate that they are almost
invisible when balsam is used with them; they are therefore usually
mounted _dry_. Others, however, are much coarser, and may be mounted in
balsam like the Diatomaceæ mentioned in Chapter III.
The common Infusoria cannot be mounted dry with any great success,
though a few may be placed upon the glass slide and allowed to dry
naturally, when their characters will be very well shown. To obtain
anything like a natural appearance, they must be put up in fluid as in
Chapter IV.
Next to the Diatomaceæ, no class of microscopic objects has been more
looked into of late than the Foraminifera. These animals are almost
all marine, having a jelly-like body enclosed in one or more chambers
of shell generally composed of carbonate of lime. The shells are made
with minute orifices, through which the pseudopodia (false feet) are
extended by which the animal is enabled to lay hold of anything and
draw itself along. From the possession of these orifices they derive
their name, as _foramen_ means a door or opening. They have been found
in every depth of sea hitherto sounded, each depth being abundant in
certain species; the lowest beds containing the greatest number of
specimens, though with less variation of kinds. In chalk they are found
in a fossil state, and may readily be shown (see Chapter III.); in
limestone and other hard stones they are abundant, and some mountains
are composed principally of these shells.
The methods of obtaining Foraminifera are various. Many may be found
upon seaweeds, which should always be examined as soon as possible
after gathering. They are found in masses upon some coasts where the
waves have carried and left them; but they are to be found the most
abundantly in sand or mud dredged from the bottom of the sea. They
must, however, be cleansed and separated from the mass of impurity
with which they are usually mixed. This may be done in various ways,
according to the nature of the accompanying matter. If sand alone, as
is frequently the case, the whole mass must be _thoroughly_ dried, and
then stirred up in clean water. The sand will soon subside by its own
weight, but the chambers of the Foraminifera, being filled with air,
will float upon the surface, and may be skimmed off. There is, however,
one objection to this mode of proceeding--some of these objects are
so minute, the chambers containing comparatively so small a quantity
of air, that they sink and are cast away with the refuse sand. On this
account it is preferable to take the trouble of searching certain
soundings under the microscope, using the camel-hair pencil, or some
other contrivance before mentioned, to extract those objects which
are required. To clean the Foraminifera, Professor Williamson advises
to transfer the specimens to an evaporating dish containing a weak
solution of caustic potash. This must be boiled for “some moments,”
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