The preparation & mounting of microscopic objects — John Shaqi
The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
After the Foraminifera and calcareous forms have been removed, the
residue may be treated with acids and levigation in the usual manner,
to obtain siliceous forms and discs, if there are any present; but to
facilitate their deposition, and to avoid the loss of any minute atoms
suspended in the washings, I would suggest the use of filtration. The
conical filter is unsuitable, as the particles would spread over too
great a surface of paper; but glass tubes open at both ends (such as
broken test-tubes) will be found to answer, the broad end covered
with filtering paper, and over that a slip of muslin tied on with a
thread to facilitate the passage of the water and prevent the risk of
breaking the paper. Suspend the tube over a suitable vessel through a
hole cut in thin wood or cardboard, pour in the washings which can be
thus filtered and then dried. The cloth must be carefully removed, the
paper cut round the edges of the tube, and the diatoms on the paper
disc may be removed by a camel-hair pencil or otherwise, ready for
mounting. Thus many objects may be preserved which would be either
washed away or only be obtained by a more tedious process.
Such is Mr. Dale’s method of cleaning the soundings from the tallow,
and as it thoroughly accomplishes its end, and is alike effective
and not injurious to Foraminifera and diatoms, it may be safely
recommended. The weak solution of caustic potash before advised for
Foraminifera, must not be used where it is desired to preserve the
diatoms, as they would certainly be injured, or destroyed altogether,
if this agent were employed.
In fixing the Foraminifera upon the slide, no better plan can be
followed than the “dry cells” and gum recommended in the early parts
of this chapter. Owing to their thickness and composition, most of
them are opaque objects only; but they are exquisitely beautiful,
and require no particular care, except in allowing the cell, &c., to
be perfectly dry, when the cover is placed upon it, or the damp will
certainly become condensed upon the inner side, and the examination
seriously interfered with.
Many of the Foraminifera require cutting into sections if it is
wished to examine the internal structure, &c.,--“decalcifying” is
also desirable in some cases;--both of these processes will be found
described at length in the chapter on Sections and Dissection.
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