The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
I have stated that the balsam is usually applied to the slide and
objects with a “bluntly-pointed glass rod;” but for the purpose of
drawing the balsam from the bottle, and conveying it to the desired
place, Dr. Carpenter uses a glass syringe with a _free_ opening. These
are his instructions:--“This (the syringe) is most readily filled with
balsam, in the first instance, by drawing out the piston, and pouring
in balsam previously rendered more liquid by gentle warmth; and nothing
else is required to enable the operator at any time to expel precisely
the amount of balsam he may require, than to warm the point of the
syringe, if the balsam should have hardened in it, and to apply a very
gentle heat to the syringe generally, if the piston should not then be
readily pressed down. When a number of balsam objects are being mounted
at one time, the advantage of this plan in regard to facility and
cleanliness (no superfluous balsam being deposited on the slide) will
make itself sensibly felt.”
When the “mounting” is thus far accomplished, the outer “wall” of
balsam may be roughly removed after a few hours have elapsed; but
great care is necessary lest the cover be moved or interfered with in
any way. In this state it may be left for the final cleansing until
the balsam becomes hard, which takes place sooner or later, according
to the degree of warmth it has been subjected to. A mantel-piece, or
some place about equal to it in temperature, is the best suited to
this purpose; and when the requisite hardness is attained, it may be
proceeded with as follows:--With a pointed knife the balsam must be
scraped away, taking care that the thin glass be not cracked by the
point getting _under_ it. If used carefully, the knife will render the
slide almost clean; but any minute portions which still adhere to the
glass must be rubbed with linen dipped in turpentine or spirit. If the
balsam is not very hard, these small fragments are readily removed by
folding a piece of paper tightly in a triangular form with many folds,
and damping the point with which the glass is rubbed. As the paper
becomes worn with the friction, the balsam will be carried off with it.
In some cases I have found this simple expedient very useful.
Public-domain text, read in full here on John Shaqi.
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